Production of a rat pancreatic polypeptide-specific monoclonal antibody and its influence on glucose homeostasis by in vivo immunoneutralization

H. C. Wong, D. K. Andersen, C. Sternini, C. L. Ruiz, E. C. Hull, J. H. Walsh, F. C. Brunicardi

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8 Scopus citations

Abstract

To test the effect of endogenous pancreatic polypeptide (PP) on rat hepatic glucose homeostasis by immunoneutralization, a rat PP-specific monoclonal antibody (MAb) was produced. Binding of this IgG1 monoclonal antibody was inhibited 50% by 350 pM rat PP. Immunohistochemistry showed that the antibody produced the expected pattern of endocrine cell staining in rat pancreas. Groups of six adult male Sprague-Dawley rats were given 5 mg of Protein-A-purified anti-PP MAb or anti-KLH MAb (control) ip every 48 hr for 5 dosing intervals. The rate of hepatic glucose output during isolated liver perfusion was 0.25 ± 0.03 mg/g/min for the PP MAb-treated rats and 0.17 ± 0.02 mg/g/min for the control group (p < 0.05). Liver glycogen content was 21.8 ± 2.9 mg/g for the PP MAb-treated rats and 14.7 ± 2.4 mg/g for the control group (N = 5). Chronic in vivo immunoneutralization of PP with this new monoclonal antibody suggests that PP influences glucose homeostasis in the rat by affecting hepatic glucose output.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalHybridoma
Volume14
Issue number4
DOIs
StatePublished - Jan 1 1995

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ASJC Scopus subject areas

  • Immunology
  • Genetics

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