Producing primate embryonic stem cells by somatic cell nuclear transfer

J. A. Byrne, D. A. Pedersen, L. L. Clepper, M. Nelson, W. G. Sanger, S. Gokhale, D. P. Wolf, Shoukhrat Mitalipov

    Research output: Contribution to journalArticle

    435 Citations (Scopus)

    Abstract

    Derivation of embryonic stem (ES) cells genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing concerns regarding rejection by the host immune system. However, the concept has only been achieved in the mouse, whereas inefficient reprogramming and poor embryonic development characterizes the results obtained in primates. Here, we used a modified SCNT approach to produce rhesus macaque blastocysts from adult skin fibroblasts, and successfully isolated two ES cell lines from these embryos. DNA analysis confirmed that nuclear DNA was identical to donor somatic cells and that mitochondrial DNA originated from oocytes. Both cell lines exhibited normal ES cell morphology, expressed key stem-cell markers, were transcriptionally similar to control ES cells and differentiated into multiple cell types in vitro and in vivo. Our results represent successful nuclear reprogramming of adult somatic cells into pluripotent ES cells and demonstrate proof-of-concept for therapeutic cloning in primates.

    Original languageEnglish (US)
    Pages (from-to)497-502
    Number of pages6
    JournalNature
    Volume450
    Issue number7169
    DOIs
    StatePublished - Nov 21 2007

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    Embryonic Stem Cells
    Primates
    Cell Line
    Pluripotent Stem Cells
    DNA
    Blastocyst
    Macaca mulatta
    Mitochondrial DNA
    Oocytes
    Embryonic Development
    Organism Cloning
    Immune System
    Stem Cells
    Embryonic Structures
    Fibroblasts
    Tissue Donors
    Skin
    Therapeutics

    ASJC Scopus subject areas

    • General

    Cite this

    Byrne, J. A., Pedersen, D. A., Clepper, L. L., Nelson, M., Sanger, W. G., Gokhale, S., ... Mitalipov, S. (2007). Producing primate embryonic stem cells by somatic cell nuclear transfer. Nature, 450(7169), 497-502. https://doi.org/10.1038/nature06357

    Producing primate embryonic stem cells by somatic cell nuclear transfer. / Byrne, J. A.; Pedersen, D. A.; Clepper, L. L.; Nelson, M.; Sanger, W. G.; Gokhale, S.; Wolf, D. P.; Mitalipov, Shoukhrat.

    In: Nature, Vol. 450, No. 7169, 21.11.2007, p. 497-502.

    Research output: Contribution to journalArticle

    Byrne, JA, Pedersen, DA, Clepper, LL, Nelson, M, Sanger, WG, Gokhale, S, Wolf, DP & Mitalipov, S 2007, 'Producing primate embryonic stem cells by somatic cell nuclear transfer', Nature, vol. 450, no. 7169, pp. 497-502. https://doi.org/10.1038/nature06357
    Byrne JA, Pedersen DA, Clepper LL, Nelson M, Sanger WG, Gokhale S et al. Producing primate embryonic stem cells by somatic cell nuclear transfer. Nature. 2007 Nov 21;450(7169):497-502. https://doi.org/10.1038/nature06357
    Byrne, J. A. ; Pedersen, D. A. ; Clepper, L. L. ; Nelson, M. ; Sanger, W. G. ; Gokhale, S. ; Wolf, D. P. ; Mitalipov, Shoukhrat. / Producing primate embryonic stem cells by somatic cell nuclear transfer. In: Nature. 2007 ; Vol. 450, No. 7169. pp. 497-502.
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