Abstract
Rat preprosomatostatin (rPPSS) is processed to two distinct end products in a tissue-specific manner. The analogous end products in anglerfish are derived from separate precursors, anglerfish preprosomatostatins-1 and -2 (a(1)PPSS and a(II)PPSS). This report reviews experiments demonstrating that in mammalian cells, the cell of expression, not precursor structure, determines the processing fate of the preprosomatostatins. A fusion precursor of a(II)PPSS and rPPSS was expressed in mammalian cell lines to determine that the amino-terminal 78 residues of rPPSS contain a sorting signal that directs the precursor into a regulated secretory pathway wherein proteolytic processing occurs. Preliminary studies of rPPSS pro-region mutations are presented that attempt to further localize this sorting signal.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 26-29 |
| Number of pages | 4 |
| Journal | Metabolism |
| Volume | 39 |
| Issue number | 9 SUPPL. 2 |
| DOIs | |
| State | Published - 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Processing and intracellular sorting of anglerfish and rat preprosomatostatins in mammalian endocrine cells. / Sevarino, Kevin A.; Ventimiglia, Roseann; Stork, Philip.
In: Metabolism, Vol. 39, No. 9 SUPPL. 2, 1990, p. 26-29.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Processing and intracellular sorting of anglerfish and rat preprosomatostatins in mammalian endocrine cells
AU - Sevarino, Kevin A.
AU - Ventimiglia, Roseann
AU - Stork, Philip
PY - 1990
Y1 - 1990
N2 - Rat preprosomatostatin (rPPSS) is processed to two distinct end products in a tissue-specific manner. The analogous end products in anglerfish are derived from separate precursors, anglerfish preprosomatostatins-1 and -2 (a(1)PPSS and a(II)PPSS). This report reviews experiments demonstrating that in mammalian cells, the cell of expression, not precursor structure, determines the processing fate of the preprosomatostatins. A fusion precursor of a(II)PPSS and rPPSS was expressed in mammalian cell lines to determine that the amino-terminal 78 residues of rPPSS contain a sorting signal that directs the precursor into a regulated secretory pathway wherein proteolytic processing occurs. Preliminary studies of rPPSS pro-region mutations are presented that attempt to further localize this sorting signal.
AB - Rat preprosomatostatin (rPPSS) is processed to two distinct end products in a tissue-specific manner. The analogous end products in anglerfish are derived from separate precursors, anglerfish preprosomatostatins-1 and -2 (a(1)PPSS and a(II)PPSS). This report reviews experiments demonstrating that in mammalian cells, the cell of expression, not precursor structure, determines the processing fate of the preprosomatostatins. A fusion precursor of a(II)PPSS and rPPSS was expressed in mammalian cell lines to determine that the amino-terminal 78 residues of rPPSS contain a sorting signal that directs the precursor into a regulated secretory pathway wherein proteolytic processing occurs. Preliminary studies of rPPSS pro-region mutations are presented that attempt to further localize this sorting signal.
UR - http://www.scopus.com/inward/record.url?scp=0025002346&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025002346&partnerID=8YFLogxK
U2 - 10.1016/0026-0495(90)90203-O
DO - 10.1016/0026-0495(90)90203-O
M3 - Article
C2 - 1976215
AN - SCOPUS:0025002346
VL - 39
SP - 26
EP - 29
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
SN - 0026-0495
IS - 9 SUPPL. 2
ER -