Probing the Effects of Gating on the Ion Occupancy of the K+ Channel Selectivity Filter Using Two-Dimensional Infrared Spectroscopy

Huong T. Kratochvil, Michał Maj, Kimberly Matulef, Alvin W. Annen, Jared Ostmeyer, Eduardo Perozo, Benoît Roux, Francis I. Valiyaveetil, Martin T. Zanni

Research output: Research - peer-reviewArticle

Abstract

The interplay between the intracellular gate and the selectivity filter underlies the structural basis for gating in potassium ion channels. Using a combination of protein semisynthesis, two-dimensional infrared (2D IR) spectroscopy, and molecular dynamics (MD) simulations, we probe the ion occupancy at the S1 binding site in the constricted state of the selectivity filter of the KcsA channel when the intracellular gate is open and closed. The 2D IR spectra resolve two features, whose relative intensities depend on the state of the intracellular gate. By matching the experiment to calculated 2D IR spectra of structures predicted by MD simulations, we identify the two features as corresponding to states with S1 occupied or unoccupied by K+. We learn that S1 is >70% occupied when the intracellular gate is closed and <15% occupied when the gate is open. Comparison of MD trajectories show that opening of the intracellular gate causes a structural change in the selectivity filter, which leads to a change in the ion occupancy. This work reveals the complexity of the conformational landscape of the K+ channel selectivity filter and its dependence on the state of the intracellular gate.

LanguageEnglish (US)
Pages8837-8845
Number of pages9
JournalJournal of the American Chemical Society
Volume139
Issue number26
DOIs
StatePublished - Jul 5 2017

Fingerprint

Molecular dynamics
Infrared spectroscopy
Ions
Molecular Dynamics Simulation
Spectrum Analysis
Computer simulation
Potassium Channels
Binding Sites
Trajectories
Proteins
Experiments
Binding sites
Potassium

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Kratochvil, H. T., Maj, M., Matulef, K., Annen, A. W., Ostmeyer, J., Perozo, E., ... Zanni, M. T. (2017). Probing the Effects of Gating on the Ion Occupancy of the K+ Channel Selectivity Filter Using Two-Dimensional Infrared Spectroscopy. Journal of the American Chemical Society, 139(26), 8837-8845. DOI: 10.1021/jacs.7b01594

Probing the Effects of Gating on the Ion Occupancy of the K+ Channel Selectivity Filter Using Two-Dimensional Infrared Spectroscopy. / Kratochvil, Huong T.; Maj, Michał; Matulef, Kimberly; Annen, Alvin W.; Ostmeyer, Jared; Perozo, Eduardo; Roux, Benoît; Valiyaveetil, Francis I.; Zanni, Martin T.

In: Journal of the American Chemical Society, Vol. 139, No. 26, 05.07.2017, p. 8837-8845.

Research output: Research - peer-reviewArticle

Kratochvil, Huong T. ; Maj, Michał ; Matulef, Kimberly ; Annen, Alvin W. ; Ostmeyer, Jared ; Perozo, Eduardo ; Roux, Benoît ; Valiyaveetil, Francis I. ; Zanni, Martin T./ Probing the Effects of Gating on the Ion Occupancy of the K+ Channel Selectivity Filter Using Two-Dimensional Infrared Spectroscopy. In: Journal of the American Chemical Society. 2017 ; Vol. 139, No. 26. pp. 8837-8845
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