Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23

Amander T. Clark, Sofia Gkountela, Di Chen, Wanlu Liu, Enrique Sosa, Meena Sukhwani, Jon Hennebold, Kyle E. Orwig

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Primordial germ cells (PGCs) are the earliest embryonic progenitors in the germline. Correct formation of PGCs is critical to reproductive health as an adult. Recent work has shown that primate PGCs can be differentiated from pluripotent stem cells; however, a bioassay that supports their identity as transplantable germ cells has not been reported. Here, we adopted a xenotransplantation assay by transplanting single-cell suspensions of human and nonhuman primate embryonic Macaca mulatta (rhesus macaque) testes containing PGCs into the seminiferous tubules of adult busulfan-treated nude mice. We discovered that both human and nonhuman primate embryonic testis are xenotransplantable, generating colonies while not generating tumors. Taken together, this work provides two critical references (molecular and functional) for defining transplantable primate PGCs. These results provide a blueprint for differentiating pluripotent stem cells to transplantable PGC-like cells in a species that is amenable to transplantation and fertility studies.

    Original languageEnglish (US)
    Pages (from-to)329-341
    Number of pages13
    JournalStem Cell Reports
    Volume9
    Issue number1
    DOIs
    StatePublished - Jul 11 2017

    Fingerprint

    Cell Transplantation
    Germ Cells
    Primates
    Cells
    Stem cells
    Pluripotent Stem Cells
    Macaca mulatta
    Blueprints
    Busulfan
    Testis
    Bioassay
    Tumors
    Assays
    Suspensions
    Heterologous Transplantation
    Seminiferous Tubules
    Reproductive Health
    Health
    Nude Mice
    Biological Assay

    Keywords

    • PGC
    • primordial-germ-cell
    • transplant
    • xenotransplant

    ASJC Scopus subject areas

    • Biochemistry
    • Genetics
    • Developmental Biology
    • Cell Biology

    Cite this

    Clark, A. T., Gkountela, S., Chen, D., Liu, W., Sosa, E., Sukhwani, M., ... Orwig, K. E. (2017). Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. Stem Cell Reports, 9(1), 329-341. https://doi.org/10.1016/j.stemcr.2017.05.002

    Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. / Clark, Amander T.; Gkountela, Sofia; Chen, Di; Liu, Wanlu; Sosa, Enrique; Sukhwani, Meena; Hennebold, Jon; Orwig, Kyle E.

    In: Stem Cell Reports, Vol. 9, No. 1, 11.07.2017, p. 329-341.

    Research output: Contribution to journalArticle

    Clark, AT, Gkountela, S, Chen, D, Liu, W, Sosa, E, Sukhwani, M, Hennebold, J & Orwig, KE 2017, 'Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23', Stem Cell Reports, vol. 9, no. 1, pp. 329-341. https://doi.org/10.1016/j.stemcr.2017.05.002
    Clark, Amander T. ; Gkountela, Sofia ; Chen, Di ; Liu, Wanlu ; Sosa, Enrique ; Sukhwani, Meena ; Hennebold, Jon ; Orwig, Kyle E. / Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. In: Stem Cell Reports. 2017 ; Vol. 9, No. 1. pp. 329-341.
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