Primary structure of the gene encoding rat preprosomatostatin

M. R. Montminy, Richard Goodman, S. J. Horovitch, J. F. Habener

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The somatostatins are peptides of 14 and 28 amino acids that are produced in a variety of endocrine and nonendocrine tissues. These peptides inhibit the secretion of many different pituitary, pancreatic, and gastrointestinal hormones. Previously, we have reported the isolation and nucleotide sequence of a cDNA derived from a rat medullary thyroid carcinoma that encoded preprosomatostatin, a 116-amino-acid precursor of somatostatin. We now report the structural characterization of the rat somatostatin gene isolated from recombinant bacteriophage libraries prepared from rat liver DNA. The gene spans 1.2 kilobases and is interrupted within the coding sequence of prosomatostatin by a single intron of 630 bases. A sequence characteristic of a Goldberg-Hogness promoter ('TATA' box), T-T-T-A-A-A-A, is located 31 bases up-stream from the transcriptional initiation site. A repetitive DNA sequence, highly reiterated in the rat genome, is located in the 5' flanking region of the gene within 900 bases of the initiation site.

Original languageEnglish (US)
Pages (from-to)3337-3340
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number11 I
StatePublished - 1984
Externally publishedYes

Fingerprint

Somatostatin
Genes
Somatostatin-28
Pancreatic Hormones
Gastrointestinal Hormones
Amino Acids
Peptides
TATA Box
Pituitary Hormones
Nucleic Acid Repetitive Sequences
5' Flanking Region
Bacteriophages
Introns
Complementary DNA
Genome
Liver
DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Primary structure of the gene encoding rat preprosomatostatin. / Montminy, M. R.; Goodman, Richard; Horovitch, S. J.; Habener, J. F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, No. 11 I, 1984, p. 3337-3340.

Research output: Contribution to journalArticle

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