Primary structure of a cardioactive neuropeptide from the tobacco hawkmoth, Manduca sexta

Clement C. Cheung; Poh Kheng Loi; Andy W. Sylwester; Terry D. Lee; Nathan J. Tublitz

doi:10.1016/0014-5793(92)81436-P

Primary structure of a cardioactive neuropeptide from the tobacco hawkmoth, Manduca sexta

Clement C. Cheung, Poh Kheng Loi, Andy W. Sylwester, Terry D. Lee, Nathan J. Tublitz

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

The amino acid sequence of the first of a family of insect cardioregulatory peptides from the tobacco hawkmoth, Manduca sexta, has been determined using a combination of Edman degradation microsequencing and mass spectroscopy. This peptide contains 9 amino acid residues and an observed mass for the monoisotopic protonated molecule of 956.4 Da. There are two cysteines at positions 3 and 9 forming a disulfide bridge and the carboxyl-terminus is amidated. The structure of this peptide, Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH₂, is identical to a peptide recently isolated from crabs called crustacean cardioactive peptide (CCAP) and we propose that this peptide be named Manduca CCAP.

Original language	English (US)
Pages (from-to)	165-168
Number of pages	4
Journal	FEBS Letters
Volume	313
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(92)81436-P
State	Published - Nov 23 1992
Externally published	Yes

Keywords

Cardioregulation
Insect neuropeptide
Invertebrate neuropeptide

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(92)81436-P

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title = "Primary structure of a cardioactive neuropeptide from the tobacco hawkmoth, Manduca sexta",

abstract = "The amino acid sequence of the first of a family of insect cardioregulatory peptides from the tobacco hawkmoth, Manduca sexta, has been determined using a combination of Edman degradation microsequencing and mass spectroscopy. This peptide contains 9 amino acid residues and an observed mass for the monoisotopic protonated molecule of 956.4 Da. There are two cysteines at positions 3 and 9 forming a disulfide bridge and the carboxyl-terminus is amidated. The structure of this peptide, Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2, is identical to a peptide recently isolated from crabs called crustacean cardioactive peptide (CCAP) and we propose that this peptide be named Manduca CCAP.",

keywords = "Cardioregulation, Insect neuropeptide, Invertebrate neuropeptide",

author = "Cheung, {Clement C.} and Loi, {Poh Kheng} and Sylwester, {Andy W.} and Lee, {Terry D.} and Tublitz, {Nathan J.}",

note = "Funding Information: AcAnowledgemem~ We are grateful for tile technical ahsBtanc¢ of Debra McMillen and the use of the l'acd~t~es of the Biotechnology Laboratory in the ln~utute of Molecular Biology at me University of Olegon. We also thank Dr. James Schilling and colleagues at the Protein Sequencing Unit of the Umversity of Cahforma, Dav~s, for their help m the sequence analy~b. Thi~ work was supported by grants from the National lnstituten of Health (NS.01258 to N J.T., and GM-40673 and DK 33155 to T D L.), Natmnal Science Foundatmn (BNS-9009155 to NJ T ), the AIIYed P. Sloan Foundation (to N,J,T.), and the Medlc.al Research Foundation of Oregon (to N.J.T.)",

year = "1992",

month = nov,

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doi = "10.1016/0014-5793(92)81436-P",

language = "English (US)",

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pages = "165--168",

journal = "FEBS Letters",

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T1 - Primary structure of a cardioactive neuropeptide from the tobacco hawkmoth, Manduca sexta

AU - Cheung, Clement C.

AU - Loi, Poh Kheng

AU - Sylwester, Andy W.

AU - Lee, Terry D.

AU - Tublitz, Nathan J.

N1 - Funding Information: AcAnowledgemem~ We are grateful for tile technical ahsBtanc¢ of Debra McMillen and the use of the l'acd~t~es of the Biotechnology Laboratory in the ln~utute of Molecular Biology at me University of Olegon. We also thank Dr. James Schilling and colleagues at the Protein Sequencing Unit of the Umversity of Cahforma, Dav~s, for their help m the sequence analy~b. Thi~ work was supported by grants from the National lnstituten of Health (NS.01258 to N J.T., and GM-40673 and DK 33155 to T D L.), Natmnal Science Foundatmn (BNS-9009155 to NJ T ), the AIIYed P. Sloan Foundation (to N,J,T.), and the Medlc.al Research Foundation of Oregon (to N.J.T.)

PY - 1992/11/23

Y1 - 1992/11/23

N2 - The amino acid sequence of the first of a family of insect cardioregulatory peptides from the tobacco hawkmoth, Manduca sexta, has been determined using a combination of Edman degradation microsequencing and mass spectroscopy. This peptide contains 9 amino acid residues and an observed mass for the monoisotopic protonated molecule of 956.4 Da. There are two cysteines at positions 3 and 9 forming a disulfide bridge and the carboxyl-terminus is amidated. The structure of this peptide, Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2, is identical to a peptide recently isolated from crabs called crustacean cardioactive peptide (CCAP) and we propose that this peptide be named Manduca CCAP.

AB - The amino acid sequence of the first of a family of insect cardioregulatory peptides from the tobacco hawkmoth, Manduca sexta, has been determined using a combination of Edman degradation microsequencing and mass spectroscopy. This peptide contains 9 amino acid residues and an observed mass for the monoisotopic protonated molecule of 956.4 Da. There are two cysteines at positions 3 and 9 forming a disulfide bridge and the carboxyl-terminus is amidated. The structure of this peptide, Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2, is identical to a peptide recently isolated from crabs called crustacean cardioactive peptide (CCAP) and we propose that this peptide be named Manduca CCAP.

KW - Cardioregulation

KW - Insect neuropeptide

KW - Invertebrate neuropeptide

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ER -

Primary structure of a cardioactive neuropeptide from the tobacco hawkmoth, Manduca sexta

Abstract

Keywords

ASJC Scopus subject areas

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