Primary structure and functional expression of a mammalian skeletal muscle sodium channel

James S. Trimmer; Sharon S. Cooperman; Sally A. Tomiko; Jiuying Zhou; Shelia M. Crean; Mary B. Boyle; Roland G. Kalen; Zuhang Sheng; Robert L. Barchi; Frederick J. Sigworth; Richard H. Goodman; William S. Agnew; Gail Mandel

doi:10.1016/0896-6273(89)90113-X

Primary structure and functional expression of a mammalian skeletal muscle sodium channel

James S. Trimmer, Sharon S. Cooperman, Sally A. Tomiko, Jiuying Zhou, Shelia M. Crean, Mary B. Boyle, Roland G. Kalen, Zuhang Sheng, Robert L. Barchi, Frederick J. Sigworth, Richard H. Goodman, William S. Agnew, Gail Mandel

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Abstract

We describe the isolation and characterization of a cDNA encoding the α subunit of a new voltage-sensitive sodium channel, μl, from rat skeletal muscle. The 1840 amino acid μl peptide is homologous to α subunits from rat brain, but, like the protein from eel electroplax, lacks an extended (∼200) amino acid segment between homologous domains I and II. Northern blot analysis indicates that the 8.5 kb μl transcript is preferentially expressed in skeletal muscle. Sodium channels expressed in Xenopus oocytes from synthetic RNA encoding μl are blocked by tetrodotoxin and μ-conotoxin at concentrations near 5 nM. The expressed sodium channels have gating kinetics similar to the native channels in rat muscle fibers, except that inactivation occurs more slowly.

Original language	English (US)
Pages (from-to)	33-49
Number of pages	17
Journal	Neuron
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/0896-6273(89)90113-X
State	Published - Jul 1989
Externally published	Yes

ASJC Scopus subject areas

Neuroscience(all)

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@article{058cb1b0503449b6a0e956a36da65e96,

title = "Primary structure and functional expression of a mammalian skeletal muscle sodium channel",

abstract = "We describe the isolation and characterization of a cDNA encoding the α subunit of a new voltage-sensitive sodium channel, μl, from rat skeletal muscle. The 1840 amino acid μl peptide is homologous to α subunits from rat brain, but, like the protein from eel electroplax, lacks an extended (∼200) amino acid segment between homologous domains I and II. Northern blot analysis indicates that the 8.5 kb μl transcript is preferentially expressed in skeletal muscle. Sodium channels expressed in Xenopus oocytes from synthetic RNA encoding μl are blocked by tetrodotoxin and μ-conotoxin at concentrations near 5 nM. The expressed sodium channels have gating kinetics similar to the native channels in rat muscle fibers, except that inactivation occurs more slowly.",

author = "Trimmer, {James S.} and Cooperman, {Sharon S.} and Tomiko, {Sally A.} and Jiuying Zhou and Crean, {Shelia M.} and Boyle, {Mary B.} and Kalen, {Roland G.} and Zuhang Sheng and Barchi, {Robert L.} and Sigworth, {Frederick J.} and Goodman, {Richard H.} and Agnew, {William S.} and Gail Mandel",

note = "Funding Information: We are grateful to Drs. Edward Moczydlowski and Baldomero M. Olivera for the generous gift of u-conotoxin CIIIA and to Dr. Matthew L. Thomas for invaluable assistance in sequence analysis, This work was supported by grants from the National Institutes of Health (#NS22518 to G. M.; #NS17928 and #HL38156 to W. S. A.) and a grant from the National Multiple Sclerosis Society (to W. S. A.). J. S. T. is supported by an NIH postdoctoral fellowship, and J. Z. is a predoctoral fellow of the Markey Foundation.",

year = "1989",

month = jul,

doi = "10.1016/0896-6273(89)90113-X",

language = "English (US)",

volume = "3",

pages = "33--49",

journal = "Neuron",

issn = "0896-6273",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Primary structure and functional expression of a mammalian skeletal muscle sodium channel

AU - Trimmer, James S.

AU - Cooperman, Sharon S.

AU - Tomiko, Sally A.

AU - Zhou, Jiuying

AU - Crean, Shelia M.

AU - Boyle, Mary B.

AU - Kalen, Roland G.

AU - Sheng, Zuhang

AU - Barchi, Robert L.

AU - Sigworth, Frederick J.

AU - Goodman, Richard H.

AU - Agnew, William S.

AU - Mandel, Gail

N1 - Funding Information: We are grateful to Drs. Edward Moczydlowski and Baldomero M. Olivera for the generous gift of u-conotoxin CIIIA and to Dr. Matthew L. Thomas for invaluable assistance in sequence analysis, This work was supported by grants from the National Institutes of Health (#NS22518 to G. M.; #NS17928 and #HL38156 to W. S. A.) and a grant from the National Multiple Sclerosis Society (to W. S. A.). J. S. T. is supported by an NIH postdoctoral fellowship, and J. Z. is a predoctoral fellow of the Markey Foundation.

PY - 1989/7

Y1 - 1989/7

N2 - We describe the isolation and characterization of a cDNA encoding the α subunit of a new voltage-sensitive sodium channel, μl, from rat skeletal muscle. The 1840 amino acid μl peptide is homologous to α subunits from rat brain, but, like the protein from eel electroplax, lacks an extended (∼200) amino acid segment between homologous domains I and II. Northern blot analysis indicates that the 8.5 kb μl transcript is preferentially expressed in skeletal muscle. Sodium channels expressed in Xenopus oocytes from synthetic RNA encoding μl are blocked by tetrodotoxin and μ-conotoxin at concentrations near 5 nM. The expressed sodium channels have gating kinetics similar to the native channels in rat muscle fibers, except that inactivation occurs more slowly.

AB - We describe the isolation and characterization of a cDNA encoding the α subunit of a new voltage-sensitive sodium channel, μl, from rat skeletal muscle. The 1840 amino acid μl peptide is homologous to α subunits from rat brain, but, like the protein from eel electroplax, lacks an extended (∼200) amino acid segment between homologous domains I and II. Northern blot analysis indicates that the 8.5 kb μl transcript is preferentially expressed in skeletal muscle. Sodium channels expressed in Xenopus oocytes from synthetic RNA encoding μl are blocked by tetrodotoxin and μ-conotoxin at concentrations near 5 nM. The expressed sodium channels have gating kinetics similar to the native channels in rat muscle fibers, except that inactivation occurs more slowly.

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VL - 3

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JO - Neuron

JF - Neuron

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Primary structure and functional expression of a mammalian skeletal muscle sodium channel

Abstract

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