Primary structure and functional expression of a mammalian skeletal muscle sodium channel

James S. Trimmer, Sharon S. Cooperman, Sally A. Tomiko, Jiuying Zhou, Shelia M. Crean, Mary B. Boyle, Roland G. Kalen, Zuhang Sheng, Robert L. Barchi, Frederick J. Sigworth, Richard Goodman, William S. Agnew, Gail Mandel

Research output: Contribution to journalArticle

461 Citations (Scopus)

Abstract

We describe the isolation and characterization of a cDNA encoding the α subunit of a new voltage-sensitive sodium channel, μl, from rat skeletal muscle. The 1840 amino acid μl peptide is homologous to α subunits from rat brain, but, like the protein from eel electroplax, lacks an extended (∼200) amino acid segment between homologous domains I and II. Northern blot analysis indicates that the 8.5 kb μl transcript is preferentially expressed in skeletal muscle. Sodium channels expressed in Xenopus oocytes from synthetic RNA encoding μl are blocked by tetrodotoxin and μ-conotoxin at concentrations near 5 nM. The expressed sodium channels have gating kinetics similar to the native channels in rat muscle fibers, except that inactivation occurs more slowly.

Original languageEnglish (US)
Pages (from-to)33-49
Number of pages17
JournalNeuron
Volume3
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

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Sodium Channels
Skeletal Muscle
Conotoxins
Amino Acids
Eels
Tetrodotoxin
Xenopus
Northern Blotting
Oocytes
Complementary DNA
RNA
Muscles
Peptides
Brain
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Trimmer, J. S., Cooperman, S. S., Tomiko, S. A., Zhou, J., Crean, S. M., Boyle, M. B., ... Mandel, G. (1989). Primary structure and functional expression of a mammalian skeletal muscle sodium channel. Neuron, 3(1), 33-49. https://doi.org/10.1016/0896-6273(89)90113-X

Primary structure and functional expression of a mammalian skeletal muscle sodium channel. / Trimmer, James S.; Cooperman, Sharon S.; Tomiko, Sally A.; Zhou, Jiuying; Crean, Shelia M.; Boyle, Mary B.; Kalen, Roland G.; Sheng, Zuhang; Barchi, Robert L.; Sigworth, Frederick J.; Goodman, Richard; Agnew, William S.; Mandel, Gail.

In: Neuron, Vol. 3, No. 1, 1989, p. 33-49.

Research output: Contribution to journalArticle

Trimmer, JS, Cooperman, SS, Tomiko, SA, Zhou, J, Crean, SM, Boyle, MB, Kalen, RG, Sheng, Z, Barchi, RL, Sigworth, FJ, Goodman, R, Agnew, WS & Mandel, G 1989, 'Primary structure and functional expression of a mammalian skeletal muscle sodium channel', Neuron, vol. 3, no. 1, pp. 33-49. https://doi.org/10.1016/0896-6273(89)90113-X
Trimmer, James S. ; Cooperman, Sharon S. ; Tomiko, Sally A. ; Zhou, Jiuying ; Crean, Shelia M. ; Boyle, Mary B. ; Kalen, Roland G. ; Sheng, Zuhang ; Barchi, Robert L. ; Sigworth, Frederick J. ; Goodman, Richard ; Agnew, William S. ; Mandel, Gail. / Primary structure and functional expression of a mammalian skeletal muscle sodium channel. In: Neuron. 1989 ; Vol. 3, No. 1. pp. 33-49.
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AU - Kalen, Roland G.

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