Primary induction of CD4 T cell responses in nasal associated lymphoid tissue during group A streptococcal infection

Haesun Park, Massimo Costalonga, R. Lee Reindhart, Priscilla E. Dombek, Marc K. Jenkins, P. Patrick Cleary

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

CD4 T cells are important for development of long-term immunity to bacterial infections. Here we describe construction of a group A streptococcus (GAS) strain that expresses the model ovalbumin epitope (OVA) on its surface, and the use of this strain in adoptive transfer experiments to study CD4 T cell response to bacterial infection in nasal-associated lymphoid tissue (NALT), which was previously shown W be a specific target for GAS colonization. The OVA+ GAS, but not the wild-type strain was shown to activate CD4 T cells in an antigen-specific manner both in vitro and in vivo. After intranasal infection of mice with this strain, OVA-specific CD4 T cells were first activated in NALT, which is functionally equivalent to human tonsils, rather than in the cervical lymph nodes. During localized infection, OVA+ GAS induced rapid and prolonged activation of CD4 T cells at higher magnitudes in the NALT than in draining lymph nodes and spleen, where CD4 T cells underwent little or no activation. In contrast, systemic infection induced significantly higher activation of CD4 T cells in both lymph nodes and spleens, compared to when the infection was localized in NALT. Further investigation of cellular immune responses in NALT during GAS infection using adoptive T cell transfer, combined with the model antigen on the pathogen may ultimately shed light on mechanisms for failure of children to develop protective immune responses following streptococcal tonsillitis.

Original languageEnglish (US)
Pages (from-to)2843-2853
Number of pages11
JournalEuropean Journal of Immunology
Volume34
Issue number10
DOIs
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

Streptococcal Infections
Lymphoid Tissue
Nose
T-Lymphocytes
Streptococcus
Ovalbumin
Epitopes
Infection
Adoptive Transfer
Lymph Nodes
Bacterial Infections
Spleen
Antigens
Tonsillitis
Palatine Tonsil
Cellular Immunity
Immunity

Keywords

  • CD4 T cells
  • GAS
  • NALT
  • Rodents

ASJC Scopus subject areas

  • Immunology

Cite this

Primary induction of CD4 T cell responses in nasal associated lymphoid tissue during group A streptococcal infection. / Park, Haesun; Costalonga, Massimo; Reindhart, R. Lee; Dombek, Priscilla E.; Jenkins, Marc K.; Cleary, P. Patrick.

In: European Journal of Immunology, Vol. 34, No. 10, 10.2004, p. 2843-2853.

Research output: Contribution to journalArticle

Park, Haesun ; Costalonga, Massimo ; Reindhart, R. Lee ; Dombek, Priscilla E. ; Jenkins, Marc K. ; Cleary, P. Patrick. / Primary induction of CD4 T cell responses in nasal associated lymphoid tissue during group A streptococcal infection. In: European Journal of Immunology. 2004 ; Vol. 34, No. 10. pp. 2843-2853.
@article{89392d69b08e4b13b45861eea74dcc64,
title = "Primary induction of CD4 T cell responses in nasal associated lymphoid tissue during group A streptococcal infection",
abstract = "CD4 T cells are important for development of long-term immunity to bacterial infections. Here we describe construction of a group A streptococcus (GAS) strain that expresses the model ovalbumin epitope (OVA) on its surface, and the use of this strain in adoptive transfer experiments to study CD4 T cell response to bacterial infection in nasal-associated lymphoid tissue (NALT), which was previously shown W be a specific target for GAS colonization. The OVA+ GAS, but not the wild-type strain was shown to activate CD4 T cells in an antigen-specific manner both in vitro and in vivo. After intranasal infection of mice with this strain, OVA-specific CD4 T cells were first activated in NALT, which is functionally equivalent to human tonsils, rather than in the cervical lymph nodes. During localized infection, OVA+ GAS induced rapid and prolonged activation of CD4 T cells at higher magnitudes in the NALT than in draining lymph nodes and spleen, where CD4 T cells underwent little or no activation. In contrast, systemic infection induced significantly higher activation of CD4 T cells in both lymph nodes and spleens, compared to when the infection was localized in NALT. Further investigation of cellular immune responses in NALT during GAS infection using adoptive T cell transfer, combined with the model antigen on the pathogen may ultimately shed light on mechanisms for failure of children to develop protective immune responses following streptococcal tonsillitis.",
keywords = "CD4 T cells, GAS, NALT, Rodents",
author = "Haesun Park and Massimo Costalonga and Reindhart, {R. Lee} and Dombek, {Priscilla E.} and Jenkins, {Marc K.} and Cleary, {P. Patrick}",
year = "2004",
month = "10",
doi = "10.1002/eji.200425242",
language = "English (US)",
volume = "34",
pages = "2843--2853",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "10",

}

TY - JOUR

T1 - Primary induction of CD4 T cell responses in nasal associated lymphoid tissue during group A streptococcal infection

AU - Park, Haesun

AU - Costalonga, Massimo

AU - Reindhart, R. Lee

AU - Dombek, Priscilla E.

AU - Jenkins, Marc K.

AU - Cleary, P. Patrick

PY - 2004/10

Y1 - 2004/10

N2 - CD4 T cells are important for development of long-term immunity to bacterial infections. Here we describe construction of a group A streptococcus (GAS) strain that expresses the model ovalbumin epitope (OVA) on its surface, and the use of this strain in adoptive transfer experiments to study CD4 T cell response to bacterial infection in nasal-associated lymphoid tissue (NALT), which was previously shown W be a specific target for GAS colonization. The OVA+ GAS, but not the wild-type strain was shown to activate CD4 T cells in an antigen-specific manner both in vitro and in vivo. After intranasal infection of mice with this strain, OVA-specific CD4 T cells were first activated in NALT, which is functionally equivalent to human tonsils, rather than in the cervical lymph nodes. During localized infection, OVA+ GAS induced rapid and prolonged activation of CD4 T cells at higher magnitudes in the NALT than in draining lymph nodes and spleen, where CD4 T cells underwent little or no activation. In contrast, systemic infection induced significantly higher activation of CD4 T cells in both lymph nodes and spleens, compared to when the infection was localized in NALT. Further investigation of cellular immune responses in NALT during GAS infection using adoptive T cell transfer, combined with the model antigen on the pathogen may ultimately shed light on mechanisms for failure of children to develop protective immune responses following streptococcal tonsillitis.

AB - CD4 T cells are important for development of long-term immunity to bacterial infections. Here we describe construction of a group A streptococcus (GAS) strain that expresses the model ovalbumin epitope (OVA) on its surface, and the use of this strain in adoptive transfer experiments to study CD4 T cell response to bacterial infection in nasal-associated lymphoid tissue (NALT), which was previously shown W be a specific target for GAS colonization. The OVA+ GAS, but not the wild-type strain was shown to activate CD4 T cells in an antigen-specific manner both in vitro and in vivo. After intranasal infection of mice with this strain, OVA-specific CD4 T cells were first activated in NALT, which is functionally equivalent to human tonsils, rather than in the cervical lymph nodes. During localized infection, OVA+ GAS induced rapid and prolonged activation of CD4 T cells at higher magnitudes in the NALT than in draining lymph nodes and spleen, where CD4 T cells underwent little or no activation. In contrast, systemic infection induced significantly higher activation of CD4 T cells in both lymph nodes and spleens, compared to when the infection was localized in NALT. Further investigation of cellular immune responses in NALT during GAS infection using adoptive T cell transfer, combined with the model antigen on the pathogen may ultimately shed light on mechanisms for failure of children to develop protective immune responses following streptococcal tonsillitis.

KW - CD4 T cells

KW - GAS

KW - NALT

KW - Rodents

UR - http://www.scopus.com/inward/record.url?scp=7244231206&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7244231206&partnerID=8YFLogxK

U2 - 10.1002/eji.200425242

DO - 10.1002/eji.200425242

M3 - Article

C2 - 15368301

AN - SCOPUS:7244231206

VL - 34

SP - 2843

EP - 2853

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 10

ER -