Primary culture of duck salt gland. I. Morphology of confluent cell layers

R. J. Lowy, J. H. Schreiber, D. C. Dawson, S. A. Ernst

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dissociated avian salt gland secretory cells were maintained in primary culture after plating on hydrated collagen gels. When seeded at 3 x 106 cells/cm2, confluent cell sheets formed within 2-3 days, whereas cultures seeded at lower densities formed a complex reticulum of cell aggregates, which remained nonconfluent even after 7 days. Scanning electron microscopy showed that the free surface of 3-day confluent cultures consisted of intermixed convex and flattened cell membranes wih prominent junctional boundaries and abundant microvilli. Transmission electron microscopy indicated that these cultures were multilayers of 1-4 cells in thickness. The plasma membranes of the superficial cells were polarized into apical and basolateral regions displaying, respectively, microvilli and interdigitating lateral membrane folds. These membrane domains were separated by shallow occluding junctions, which consisted of both single strands and simple net-like arrays in freeze-fracture images. Underlying epithelial cells retained lateral membrane folds and formed desmosomal contacts with superficial and neighboring cells. Theses cultures, unlike the intact tissue, allow direct access to the apical and basolateral cell surfaces for electrophysiological analysis of transmural active ion transport.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume18
Issue number1
StatePublished - 1985
Externally publishedYes

Fingerprint

Salt Gland
Ducks
Salts
Cells
Cell membranes
Membranes
Cell culture
Plating
Multilayers
Collagen
Microvilli
Gels
Ions
Tissue
Transmission electron microscopy
Scanning electron microscopy
Cell Membrane
Reticulum
Tight Junctions
Ion Transport

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Primary culture of duck salt gland. I. Morphology of confluent cell layers. / Lowy, R. J.; Schreiber, J. H.; Dawson, D. C.; Ernst, S. A.

In: American Journal of Physiology - Cell Physiology, Vol. 18, No. 1, 1985.

Research output: Contribution to journalArticle

Lowy, R. J. ; Schreiber, J. H. ; Dawson, D. C. ; Ernst, S. A. / Primary culture of duck salt gland. I. Morphology of confluent cell layers. In: American Journal of Physiology - Cell Physiology. 1985 ; Vol. 18, No. 1.
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