Previous Cryopreservation Alters the Natural History of the Red Blood Cell Storage Lesion

Alex L. Chang, Richard S. Hoehn, Peter Jernigan, Daniel Cox, Martin Schreiber, Timothy A. Pritts

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    BACKGROUND:: During storage, packed red blood cells (pRBCs) undergo a number of biochemical, metabolic and morphologic changes, collectively known as the “storage lesion”. We aimed to determine the effect of cryopreservation on the red blood cell storage lesion compared to traditional 4?°C storage. METHODS:: Previously cryopreserved human packed red blood cells were compared to age matched never frozen packed red blood cells obtained from the local blood bank. The development of the red cell storage lesion was evaluated after 7, 14, 21, 28, and 42 days of storage at 4?°C in AS-3 storage medium. We measured physiological parameters including cell counts, lactic acid and potassium concentrations as well as signs of eryptosis including loss of phosphatidylserine (PS) asymmetry, microparticle production and osmotic fragility in hypotonic saline. RESULTS:: Compared to controls, previously cryopreserved pRBC at 7 days of storage in AS-3 showed lower red cell counts (3.7 vs 5.3?×?10^6?cells/uL, p(

    Original languageEnglish (US)
    JournalShock
    DOIs
    StateAccepted/In press - Jul 1 2016

    Fingerprint

    Cryopreservation
    Natural History
    Erythrocytes
    Cell Count
    Osmotic Fragility
    Blood Banks
    Phosphatidylserines
    Lactic Acid
    Potassium

    ASJC Scopus subject areas

    • Critical Care and Intensive Care Medicine
    • Emergency Medicine

    Cite this

    Previous Cryopreservation Alters the Natural History of the Red Blood Cell Storage Lesion. / Chang, Alex L.; Hoehn, Richard S.; Jernigan, Peter; Cox, Daniel; Schreiber, Martin; Pritts, Timothy A.

    In: Shock, 01.07.2016.

    Research output: Contribution to journalArticle

    Chang, Alex L. ; Hoehn, Richard S. ; Jernigan, Peter ; Cox, Daniel ; Schreiber, Martin ; Pritts, Timothy A. / Previous Cryopreservation Alters the Natural History of the Red Blood Cell Storage Lesion. In: Shock. 2016.
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    AU - Schreiber, Martin

    AU - Pritts, Timothy A.

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