TY - JOUR
T1 - Prevention of the murine model of biliary atresia after live rotavirus vaccination of dams
AU - Bondoc, Alexander J.
AU - Jafri, Mubeen A.
AU - Donnelly, Bryan
AU - Mohanty, Sujit K.
AU - McNeal, Monica M.
AU - Ward, Richard L.
AU - Tiao, Greg M.
N1 - Funding Information:
We thank Jorge A. Bezerra, MD, for his critical review of the manuscript. This project was supported in part by PHS Grant P30DK078392.
PY - 2009/8
Y1 - 2009/8
N2 - Purpose: Biliary atresia (BA) is a neonatal disease that results in the obliteration of the biliary tree. The murine model of BA has been established where rhesus rotavirus (RRV) infection of newborn mice leads to an obstructive cholangiopathy. We determined whether maternal postconception rotavirus vaccination could prevent the murine model of BA. Materials and Methods: Female mice were mated and injected intraperitoneally with one of the following materials: purified rotavirus strains RRV or Wa, high or low-dose Rotateq (Merck and Co Inc, Whitehouse Station, NJ) (a pentavalent rotavirus vaccine [PRV]), purified recombinant viral antigens of rotavirus (VP6) or influenza (NP), or saline. B-cell-deficient females also underwent postconception PRV injection. Results: Maternal vaccination with PRV improves survival of pups infected with RRV. Serum rotavirus IgG, but not IgA, levels were increased in pups delivered from dams who received RRV, Wa, PRV, or VP6, but in the case of the Wa, PRV, and VP6 groups, these antibodies were not neutralizing. Postconception injection of high-dose PRV did not improve survival of pups born to B-cell-deficient dams. Conclusion: Maternal vaccination against RRV can prevent the rotavirus-induced murine model of BA in newborn mouse pups.
AB - Purpose: Biliary atresia (BA) is a neonatal disease that results in the obliteration of the biliary tree. The murine model of BA has been established where rhesus rotavirus (RRV) infection of newborn mice leads to an obstructive cholangiopathy. We determined whether maternal postconception rotavirus vaccination could prevent the murine model of BA. Materials and Methods: Female mice were mated and injected intraperitoneally with one of the following materials: purified rotavirus strains RRV or Wa, high or low-dose Rotateq (Merck and Co Inc, Whitehouse Station, NJ) (a pentavalent rotavirus vaccine [PRV]), purified recombinant viral antigens of rotavirus (VP6) or influenza (NP), or saline. B-cell-deficient females also underwent postconception PRV injection. Results: Maternal vaccination with PRV improves survival of pups infected with RRV. Serum rotavirus IgG, but not IgA, levels were increased in pups delivered from dams who received RRV, Wa, PRV, or VP6, but in the case of the Wa, PRV, and VP6 groups, these antibodies were not neutralizing. Postconception injection of high-dose PRV did not improve survival of pups born to B-cell-deficient dams. Conclusion: Maternal vaccination against RRV can prevent the rotavirus-induced murine model of BA in newborn mouse pups.
KW - Biliary atresia
KW - Maternal immunization
KW - Rotateq
KW - Rotavirus
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U2 - 10.1016/j.jpedsurg.2009.05.034
DO - 10.1016/j.jpedsurg.2009.05.034
M3 - Article
C2 - 19635292
AN - SCOPUS:67650935178
SN - 0022-3468
VL - 44
SP - 1479
EP - 1490
JO - Journal of pediatric surgery
JF - Journal of pediatric surgery
IS - 8
ER -