TY - JOUR
T1 - Prevalence of Enterococcus faecalis at multiple oral sites in endodontic patients using culture and PCR
AU - Sedgley, Christine
AU - Buck, Gwendolyn
AU - Appelbe, Oliver
N1 - Funding Information:
This study was supported by the Endodontic Research Fund, University of Michigan and by a grant made available by the University of Michigan to the Student Research Program.
PY - 2006/2
Y1 - 2006/2
N2 - There are conflicting data on the occurrence of Enterococcus faecalis in the oral cavity of endodontic patients. This study investigated the prevalence of E. faecalis in multi-site oral samples (n = 136) from 41 endodontic patients using culture and polymerase chain reaction (PCR). Additionally, culturable strains were investigated for virulence traits. Overall, E. faecalis was detected in at least one tongue, oral rinse, or gingival sulcus sample in 68% of patients and in the root canals only in 5% of patients. In 21 patients from whom samples were obtained from all four sites, E. faecalis was detected in more tongue than gingival sulcus, oral rinse, and root canal samples (43, 14, 10, and 10%, respectively; p = 0.0148, χ2), and in proportionally greater numbers of patients with gingivitis/periodontitis compared to healthy periodontium (73% versus 20%; p = 0.03, Fisher's exact test). PCR was more sensitive than culture in detecting E. faecalis in oral samples (32% and 4%, respectively; p < 0.0001, McNemar's test). Multiple virulence traits were identified in culturable strains.
AB - There are conflicting data on the occurrence of Enterococcus faecalis in the oral cavity of endodontic patients. This study investigated the prevalence of E. faecalis in multi-site oral samples (n = 136) from 41 endodontic patients using culture and polymerase chain reaction (PCR). Additionally, culturable strains were investigated for virulence traits. Overall, E. faecalis was detected in at least one tongue, oral rinse, or gingival sulcus sample in 68% of patients and in the root canals only in 5% of patients. In 21 patients from whom samples were obtained from all four sites, E. faecalis was detected in more tongue than gingival sulcus, oral rinse, and root canal samples (43, 14, 10, and 10%, respectively; p = 0.0148, χ2), and in proportionally greater numbers of patients with gingivitis/periodontitis compared to healthy periodontium (73% versus 20%; p = 0.03, Fisher's exact test). PCR was more sensitive than culture in detecting E. faecalis in oral samples (32% and 4%, respectively; p < 0.0001, McNemar's test). Multiple virulence traits were identified in culturable strains.
KW - Culturing
KW - Endodontic
KW - Enterococcus faecalis
KW - Oral
KW - PCR
KW - Virulence
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U2 - 10.1016/j.joen.2005.10.022
DO - 10.1016/j.joen.2005.10.022
M3 - Article
C2 - 16427455
AN - SCOPUS:33644587732
SN - 0099-2399
VL - 32
SP - 104
EP - 109
JO - Journal of endodontics
JF - Journal of endodontics
IS - 2
ER -