Prevalence & susceptibility to fluconazole of Candida species causing vulvovaginitis

Srujana Mohanty, Immaculata Xess, Fahmi Hasan, Arti Kapil, Suneeta Mittal, Jorge Tolosa

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Background & objectives: Vulvovaginal candidiasis is an important cause of morbidity in women of reproductive age. This study was carried out to determine the species prevalence and susceptibility pattern to fluconazole of yeasts isolated from the vagina of symptomatic women. Methods: This prospective study was conducted in a rural primary health care center of north India from May 2003 to April 2004 and included 601 married, sexually active women (18-49 yr) with the self reported symptoms of vaginal discharge and/or genital itching and/or genital burning. Specific aetiology of the genitourinary symptoms including candidal infection were determined. Specimens from the lateral wall of vagina were subjected !o direct wet mount microscopy and fungal culture on Sabouraud's dextrose agar. Susceptibility testing to fluconazole was carried out using broth microdilution method. Results: Yeasts were isolated in 111 (18.5%) women and these consisted of Candida glabrata (56, 50.4%), C. albicans (39, 35.1%), C. tropicalis (12, 10.8%), C. krusei (3, 2.7%) and C. parapsilosis (1, 0.9%). Susceptibility testing carried out on 30 representative isolates (15 C. glabrata, 10 C. albicans, 4 C. tropicalis and 1 C. parapsilosis) revealed that 21 isolates (70%) were susceptible (MIC, ≤ 8 μg/ml) to fluconazole while 9 (30%) were susceptible-dose dependent (S-DD, MIC 16-32 μg/ml). Interpretation & conclusion: Our findings suggest a low prevalence of fluconazole resistance in vaginal candida isolates in our population. However, a high prevalence of non-albicans candida species and increased dose-dependent resistance in these isolates necessitates vigilance since this may warrant a change in the optimal therapy of non-albicans candida vaginitis.

Original languageEnglish (US)
Pages (from-to)216-219
Number of pages4
JournalIndian Journal of Medical Research
Volume126
Issue number3
StatePublished - Sep 2007
Externally publishedYes

Fingerprint

Vulvovaginitis
Fluconazole
Candida
Candida glabrata
Vagina
Yeast
Yeasts
Vulvovaginal Candidiasis
Vaginitis
Vaginal Discharge
Rural Health
Testing
Pruritus
Health care
Agar
India
Microscopy
Primary Health Care
Microscopic examination
Prospective Studies

Keywords

  • Antifungal susceptibility
  • Candida
  • Candida vaginitis
  • Fluconazole
  • Non-albicans species

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Prevalence & susceptibility to fluconazole of Candida species causing vulvovaginitis. / Mohanty, Srujana; Xess, Immaculata; Hasan, Fahmi; Kapil, Arti; Mittal, Suneeta; Tolosa, Jorge.

In: Indian Journal of Medical Research, Vol. 126, No. 3, 09.2007, p. 216-219.

Research output: Contribution to journalArticle

Mohanty, S, Xess, I, Hasan, F, Kapil, A, Mittal, S & Tolosa, J 2007, 'Prevalence & susceptibility to fluconazole of Candida species causing vulvovaginitis', Indian Journal of Medical Research, vol. 126, no. 3, pp. 216-219.
Mohanty, Srujana ; Xess, Immaculata ; Hasan, Fahmi ; Kapil, Arti ; Mittal, Suneeta ; Tolosa, Jorge. / Prevalence & susceptibility to fluconazole of Candida species causing vulvovaginitis. In: Indian Journal of Medical Research. 2007 ; Vol. 126, No. 3. pp. 216-219.
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AB - Background & objectives: Vulvovaginal candidiasis is an important cause of morbidity in women of reproductive age. This study was carried out to determine the species prevalence and susceptibility pattern to fluconazole of yeasts isolated from the vagina of symptomatic women. Methods: This prospective study was conducted in a rural primary health care center of north India from May 2003 to April 2004 and included 601 married, sexually active women (18-49 yr) with the self reported symptoms of vaginal discharge and/or genital itching and/or genital burning. Specific aetiology of the genitourinary symptoms including candidal infection were determined. Specimens from the lateral wall of vagina were subjected !o direct wet mount microscopy and fungal culture on Sabouraud's dextrose agar. Susceptibility testing to fluconazole was carried out using broth microdilution method. Results: Yeasts were isolated in 111 (18.5%) women and these consisted of Candida glabrata (56, 50.4%), C. albicans (39, 35.1%), C. tropicalis (12, 10.8%), C. krusei (3, 2.7%) and C. parapsilosis (1, 0.9%). Susceptibility testing carried out on 30 representative isolates (15 C. glabrata, 10 C. albicans, 4 C. tropicalis and 1 C. parapsilosis) revealed that 21 isolates (70%) were susceptible (MIC, ≤ 8 μg/ml) to fluconazole while 9 (30%) were susceptible-dose dependent (S-DD, MIC 16-32 μg/ml). Interpretation & conclusion: Our findings suggest a low prevalence of fluconazole resistance in vaginal candida isolates in our population. However, a high prevalence of non-albicans candida species and increased dose-dependent resistance in these isolates necessitates vigilance since this may warrant a change in the optimal therapy of non-albicans candida vaginitis.

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