Preterm labor

Stimulation of arachidonic acid metabolism in human amnion cells by bacterial products

Phillip R. Bennett, Mathew P. Rose, Leslie Myatt, Murdoch G. Elder

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

There is a strong association between preterm labor and infection. Some potentially pathogenic bacteria have phospholipase activity, and it has been suggested that release of phospholipase from these organisms may increase prostaglandin E2 synthesis in amnion cells and hence initiate preterm labor. In this study we established monolayer amnion cell cultures from tissue collected at elective cesarean section at term before labor. Cells were prelabeled with tritiated arachidonic acid and then further incubated after addition of 2%, 5%, or 10% (vol/vol) filtered medium in which either group B β-hemolytic streptococcus, Streptococcus viridans, Escherichia coli, Bacteroides fragilis, or Lactobacillus had been growing. Tritiated arachidonic acid and its metabolites released by the amnion cells in these or control incubates were extracted from culture medium and separated by high-performance liquid chromatography. Addition of conditioned medium from each of the organisms with the exception of Lactobacillus caused an increase in overall arachidonic acid metabolism. There was an increase in the ratio of cyclooxygenase to lipoxgenase metabolism and in prostaglandin E2 production in particular when compared to controls. The profile of arachidonic acid metabolism in amnion cells following addition of filtered bacterial medium resembled that obtained from amnion cells cultured following spontaneous labor. We suggest that abnormal bacterial colonization of the genital tract may lead to an increase in arachidonic acid metabolism in amnion cells with an increase in prostaglandin E2 production and the consequent initiation of preterm labor.

Original languageEnglish (US)
Pages (from-to)649-655
Number of pages7
JournalAmerican Journal of Obstetrics and Gynecology
Volume156
Issue number3
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Amnion
Premature Obstetric Labor
Arachidonic Acid
Dinoprostone
Phospholipases
Lactobacillus
Viridans Streptococci
Bacteroides fragilis
Streptococcus agalactiae
Prostaglandin-Endoperoxide Synthases
Conditioned Culture Medium
Cesarean Section
Culture Media
Cultured Cells
Cell Culture Techniques
High Pressure Liquid Chromatography
Escherichia coli
Bacteria
Infection

Keywords

  • Amnion
  • bacteria
  • preterm labor
  • prostaglandins

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Preterm labor : Stimulation of arachidonic acid metabolism in human amnion cells by bacterial products. / Bennett, Phillip R.; Rose, Mathew P.; Myatt, Leslie; Elder, Murdoch G.

In: American Journal of Obstetrics and Gynecology, Vol. 156, No. 3, 1987, p. 649-655.

Research output: Contribution to journalArticle

@article{2084c97fe4954da78cffa122e96ea67f,
title = "Preterm labor: Stimulation of arachidonic acid metabolism in human amnion cells by bacterial products",
abstract = "There is a strong association between preterm labor and infection. Some potentially pathogenic bacteria have phospholipase activity, and it has been suggested that release of phospholipase from these organisms may increase prostaglandin E2 synthesis in amnion cells and hence initiate preterm labor. In this study we established monolayer amnion cell cultures from tissue collected at elective cesarean section at term before labor. Cells were prelabeled with tritiated arachidonic acid and then further incubated after addition of 2{\%}, 5{\%}, or 10{\%} (vol/vol) filtered medium in which either group B β-hemolytic streptococcus, Streptococcus viridans, Escherichia coli, Bacteroides fragilis, or Lactobacillus had been growing. Tritiated arachidonic acid and its metabolites released by the amnion cells in these or control incubates were extracted from culture medium and separated by high-performance liquid chromatography. Addition of conditioned medium from each of the organisms with the exception of Lactobacillus caused an increase in overall arachidonic acid metabolism. There was an increase in the ratio of cyclooxygenase to lipoxgenase metabolism and in prostaglandin E2 production in particular when compared to controls. The profile of arachidonic acid metabolism in amnion cells following addition of filtered bacterial medium resembled that obtained from amnion cells cultured following spontaneous labor. We suggest that abnormal bacterial colonization of the genital tract may lead to an increase in arachidonic acid metabolism in amnion cells with an increase in prostaglandin E2 production and the consequent initiation of preterm labor.",
keywords = "Amnion, bacteria, preterm labor, prostaglandins",
author = "Bennett, {Phillip R.} and Rose, {Mathew P.} and Leslie Myatt and Elder, {Murdoch G.}",
year = "1987",
doi = "10.1016/0002-9378(87)90070-6",
language = "English (US)",
volume = "156",
pages = "649--655",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "3",

}

TY - JOUR

T1 - Preterm labor

T2 - Stimulation of arachidonic acid metabolism in human amnion cells by bacterial products

AU - Bennett, Phillip R.

AU - Rose, Mathew P.

AU - Myatt, Leslie

AU - Elder, Murdoch G.

PY - 1987

Y1 - 1987

N2 - There is a strong association between preterm labor and infection. Some potentially pathogenic bacteria have phospholipase activity, and it has been suggested that release of phospholipase from these organisms may increase prostaglandin E2 synthesis in amnion cells and hence initiate preterm labor. In this study we established monolayer amnion cell cultures from tissue collected at elective cesarean section at term before labor. Cells were prelabeled with tritiated arachidonic acid and then further incubated after addition of 2%, 5%, or 10% (vol/vol) filtered medium in which either group B β-hemolytic streptococcus, Streptococcus viridans, Escherichia coli, Bacteroides fragilis, or Lactobacillus had been growing. Tritiated arachidonic acid and its metabolites released by the amnion cells in these or control incubates were extracted from culture medium and separated by high-performance liquid chromatography. Addition of conditioned medium from each of the organisms with the exception of Lactobacillus caused an increase in overall arachidonic acid metabolism. There was an increase in the ratio of cyclooxygenase to lipoxgenase metabolism and in prostaglandin E2 production in particular when compared to controls. The profile of arachidonic acid metabolism in amnion cells following addition of filtered bacterial medium resembled that obtained from amnion cells cultured following spontaneous labor. We suggest that abnormal bacterial colonization of the genital tract may lead to an increase in arachidonic acid metabolism in amnion cells with an increase in prostaglandin E2 production and the consequent initiation of preterm labor.

AB - There is a strong association between preterm labor and infection. Some potentially pathogenic bacteria have phospholipase activity, and it has been suggested that release of phospholipase from these organisms may increase prostaglandin E2 synthesis in amnion cells and hence initiate preterm labor. In this study we established monolayer amnion cell cultures from tissue collected at elective cesarean section at term before labor. Cells were prelabeled with tritiated arachidonic acid and then further incubated after addition of 2%, 5%, or 10% (vol/vol) filtered medium in which either group B β-hemolytic streptococcus, Streptococcus viridans, Escherichia coli, Bacteroides fragilis, or Lactobacillus had been growing. Tritiated arachidonic acid and its metabolites released by the amnion cells in these or control incubates were extracted from culture medium and separated by high-performance liquid chromatography. Addition of conditioned medium from each of the organisms with the exception of Lactobacillus caused an increase in overall arachidonic acid metabolism. There was an increase in the ratio of cyclooxygenase to lipoxgenase metabolism and in prostaglandin E2 production in particular when compared to controls. The profile of arachidonic acid metabolism in amnion cells following addition of filtered bacterial medium resembled that obtained from amnion cells cultured following spontaneous labor. We suggest that abnormal bacterial colonization of the genital tract may lead to an increase in arachidonic acid metabolism in amnion cells with an increase in prostaglandin E2 production and the consequent initiation of preterm labor.

KW - Amnion

KW - bacteria

KW - preterm labor

KW - prostaglandins

UR - http://www.scopus.com/inward/record.url?scp=0023091017&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023091017&partnerID=8YFLogxK

U2 - 10.1016/0002-9378(87)90070-6

DO - 10.1016/0002-9378(87)90070-6

M3 - Article

VL - 156

SP - 649

EP - 655

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 3

ER -