TY - JOUR
T1 - Presynaptic HCN Channels Regulate Vesicular Glutamate Transport
AU - Huang, Hai
AU - Trussell, Laurence O.
N1 - Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/10/22
Y1 - 2014/10/22
N2 - The amount of neurotransmitter stored in synaptic vesicles determines postsynaptic quantal size and thus the strength of synaptic transmission. However, little is known about regulation of vesicular neurotransmitter uptake. In recordings from the calyx of Held, a giant mammalian glutamatergic synapse, we found that changes in presynaptic Na+ concentration above and below a resting value of 13mM regulated vesicular glutamate uptake, consistent with activation of a vesicular monovalent cation Na+(K+)/H+ exchanger. Na+ flux through presynaptic plasma membrane hyperpolarization-activated cyclic nucleotide-gated (HCN) channels enhanced presynaptic Na+ concentration and thus controlled postsynaptic quantal size. Our results indicate that a plasma membrane ion channel controls synaptic strength by modulating vesicular neurotransmitter uptake through a Na+-dependent process.
AB - The amount of neurotransmitter stored in synaptic vesicles determines postsynaptic quantal size and thus the strength of synaptic transmission. However, little is known about regulation of vesicular neurotransmitter uptake. In recordings from the calyx of Held, a giant mammalian glutamatergic synapse, we found that changes in presynaptic Na+ concentration above and below a resting value of 13mM regulated vesicular glutamate uptake, consistent with activation of a vesicular monovalent cation Na+(K+)/H+ exchanger. Na+ flux through presynaptic plasma membrane hyperpolarization-activated cyclic nucleotide-gated (HCN) channels enhanced presynaptic Na+ concentration and thus controlled postsynaptic quantal size. Our results indicate that a plasma membrane ion channel controls synaptic strength by modulating vesicular neurotransmitter uptake through a Na+-dependent process.
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U2 - 10.1016/j.neuron.2014.08.046
DO - 10.1016/j.neuron.2014.08.046
M3 - Article
C2 - 25263752
AN - SCOPUS:84908244950
SN - 0896-6273
VL - 84
SP - 340
EP - 346
JO - Neuron
JF - Neuron
IS - 2
ER -