Pressure effects on Clostridium strains isolated from a cold deep-sea environment

Federico M. Lauro; Giulio Bertoloni; Anna Obraztsova; Chiaki Kato; Bradley M. Tebo; Douglas H. Bartlett

doi:10.1007/s00792-003-0367-y

Pressure effects on Clostridium strains isolated from a cold deep-sea environment

Federico M. Lauro, Giulio Bertoloni, Anna Obraztsova, Chiaki Kato, Bradley M. Tebo, Douglas H. Bartlett

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Three Clostridium strains were isolated from deep-sea sediments collected at a depth of 6.3-7.3 km in the Japan Trench. Physiological characterization and 16S rDNA analysis revealed that the three isolates were all closely related to Clostridium bifermentans. The spores of all three isolates were resistant to inactivation at high pressure and low temperature. However, despite the fact that the vegetative cells were halotolerant and eurythermal they did not appear to be adapted for growth or viability under the conditions prevailing in the deep-sea sediments from which they were obtained. The results suggest that the isolates had survived as spores in the deep-sea sediments and that the marine benthos could be a source of clostridia originating in other environments.

Original language	English (US)
Pages (from-to)	169-173
Number of pages	5
Journal	Extremophiles
Volume	8
Issue number	2
DOIs	https://doi.org/10.1007/s00792-003-0367-y
State	Published - Apr 2004
Externally published	Yes

Keywords

Clostridium
Clostridium bifermentans
Deep sea
Japan Trench
Pressure

ASJC Scopus subject areas

Microbiology
Molecular Medicine

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10.1007/s00792-003-0367-y

Cite this

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title = "Pressure effects on Clostridium strains isolated from a cold deep-sea environment",

abstract = "Three Clostridium strains were isolated from deep-sea sediments collected at a depth of 6.3-7.3 km in the Japan Trench. Physiological characterization and 16S rDNA analysis revealed that the three isolates were all closely related to Clostridium bifermentans. The spores of all three isolates were resistant to inactivation at high pressure and low temperature. However, despite the fact that the vegetative cells were halotolerant and eurythermal they did not appear to be adapted for growth or viability under the conditions prevailing in the deep-sea sediments from which they were obtained. The results suggest that the isolates had survived as spores in the deep-sea sediments and that the marine benthos could be a source of clostridia originating in other environments.",

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T1 - Pressure effects on Clostridium strains isolated from a cold deep-sea environment

AU - Lauro, Federico M.

AU - Bertoloni, Giulio

AU - Obraztsova, Anna

AU - Kato, Chiaki

AU - Tebo, Bradley M.

AU - Bartlett, Douglas H.

PY - 2004/4

Y1 - 2004/4

N2 - Three Clostridium strains were isolated from deep-sea sediments collected at a depth of 6.3-7.3 km in the Japan Trench. Physiological characterization and 16S rDNA analysis revealed that the three isolates were all closely related to Clostridium bifermentans. The spores of all three isolates were resistant to inactivation at high pressure and low temperature. However, despite the fact that the vegetative cells were halotolerant and eurythermal they did not appear to be adapted for growth or viability under the conditions prevailing in the deep-sea sediments from which they were obtained. The results suggest that the isolates had survived as spores in the deep-sea sediments and that the marine benthos could be a source of clostridia originating in other environments.

AB - Three Clostridium strains were isolated from deep-sea sediments collected at a depth of 6.3-7.3 km in the Japan Trench. Physiological characterization and 16S rDNA analysis revealed that the three isolates were all closely related to Clostridium bifermentans. The spores of all three isolates were resistant to inactivation at high pressure and low temperature. However, despite the fact that the vegetative cells were halotolerant and eurythermal they did not appear to be adapted for growth or viability under the conditions prevailing in the deep-sea sediments from which they were obtained. The results suggest that the isolates had survived as spores in the deep-sea sediments and that the marine benthos could be a source of clostridia originating in other environments.

KW - Clostridium

KW - Clostridium bifermentans

KW - Deep sea

KW - Japan Trench

KW - Pressure

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Pressure effects on Clostridium strains isolated from a cold deep-sea environment

Abstract

Keywords

ASJC Scopus subject areas

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