Presence of hemoglobin messenger ribonucleoprotein in a reticulocyte supernatant fraction

George D. Olsen; Pauline Gaskill; David Kabat

doi:10.1016/0005-2787(72)90253-5

Presence of hemoglobin messenger ribonucleoprotein in a reticulocyte supernatant fraction

George D. Olsen, Pauline Gaskill, David Kabat

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A study is made of globin messenger ribonucleic acid (mRNA) distribution in a rabbit reticulocyte lysate. Ribonucleic acid with the electrophoretic mobility of globin mRNA is found not only in polyribosomes but also in a ribonucleoprotein complex which sediments at 15 S. Such presumptive mRNA is not found in single ribosomes or in the subribosomal particle fraction. Further evidence that the 15-S ribonucleoprotein contains globin mRNA is obtained using a protein synthesizing extract from embryonic muscle. Addition of 15-S complex to the extract specifically stimulates incorporation of radioactive amino acids into material which cochromatographs with carrier hemoglobin and which coprecipitates with the carrier hemoglobin upon addition of anti-hemoglobin antiserum.

Original language	English (US)
Pages (from-to)	297-304
Number of pages	8
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	272
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(72)90253-5
State	Published - Jun 30 1972

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title = "Presence of hemoglobin messenger ribonucleoprotein in a reticulocyte supernatant fraction",

abstract = "A study is made of globin messenger ribonucleic acid (mRNA) distribution in a rabbit reticulocyte lysate. Ribonucleic acid with the electrophoretic mobility of globin mRNA is found not only in polyribosomes but also in a ribonucleoprotein complex which sediments at 15 S. Such presumptive mRNA is not found in single ribosomes or in the subribosomal particle fraction. Further evidence that the 15-S ribonucleoprotein contains globin mRNA is obtained using a protein synthesizing extract from embryonic muscle. Addition of 15-S complex to the extract specifically stimulates incorporation of radioactive amino acids into material which cochromatographs with carrier hemoglobin and which coprecipitates with the carrier hemoglobin upon addition of anti-hemoglobin antiserum.",

author = "Olsen, {George D.} and Pauline Gaskill and David Kabat",

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T1 - Presence of hemoglobin messenger ribonucleoprotein in a reticulocyte supernatant fraction

AU - Olsen, George D.

AU - Gaskill, Pauline

AU - Kabat, David

N1 - Funding Information: This research was supported by U.S. Public Health Service Grant CA-II347.

PY - 1972/6/30

Y1 - 1972/6/30

N2 - A study is made of globin messenger ribonucleic acid (mRNA) distribution in a rabbit reticulocyte lysate. Ribonucleic acid with the electrophoretic mobility of globin mRNA is found not only in polyribosomes but also in a ribonucleoprotein complex which sediments at 15 S. Such presumptive mRNA is not found in single ribosomes or in the subribosomal particle fraction. Further evidence that the 15-S ribonucleoprotein contains globin mRNA is obtained using a protein synthesizing extract from embryonic muscle. Addition of 15-S complex to the extract specifically stimulates incorporation of radioactive amino acids into material which cochromatographs with carrier hemoglobin and which coprecipitates with the carrier hemoglobin upon addition of anti-hemoglobin antiserum.

AB - A study is made of globin messenger ribonucleic acid (mRNA) distribution in a rabbit reticulocyte lysate. Ribonucleic acid with the electrophoretic mobility of globin mRNA is found not only in polyribosomes but also in a ribonucleoprotein complex which sediments at 15 S. Such presumptive mRNA is not found in single ribosomes or in the subribosomal particle fraction. Further evidence that the 15-S ribonucleoprotein contains globin mRNA is obtained using a protein synthesizing extract from embryonic muscle. Addition of 15-S complex to the extract specifically stimulates incorporation of radioactive amino acids into material which cochromatographs with carrier hemoglobin and which coprecipitates with the carrier hemoglobin upon addition of anti-hemoglobin antiserum.

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Presence of hemoglobin messenger ribonucleoprotein in a reticulocyte supernatant fraction

Abstract

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