Presence of hemoglobin messenger ribonucleoprotein in a reticulocyte supernatant fraction

George D. Olsen, Pauline Gaskill, David Kabat

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A study is made of globin messenger ribonucleic acid (mRNA) distribution in a rabbit reticulocyte lysate. Ribonucleic acid with the electrophoretic mobility of globin mRNA is found not only in polyribosomes but also in a ribonucleoprotein complex which sediments at 15 S. Such presumptive mRNA is not found in single ribosomes or in the subribosomal particle fraction. Further evidence that the 15-S ribonucleoprotein contains globin mRNA is obtained using a protein synthesizing extract from embryonic muscle. Addition of 15-S complex to the extract specifically stimulates incorporation of radioactive amino acids into material which cochromatographs with carrier hemoglobin and which coprecipitates with the carrier hemoglobin upon addition of anti-hemoglobin antiserum.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume272
Issue number2
DOIs
StatePublished - Jun 30 1972
Externally publishedYes

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Hemoglobins
Globins
RNA
Ribonucleoproteins
Electrophoretic mobility
Muscle
Immune Sera
Sediments
messenger ribonucleoprotein
Amino Acids
Proteins

Cite this

Presence of hemoglobin messenger ribonucleoprotein in a reticulocyte supernatant fraction. / Olsen, George D.; Gaskill, Pauline; Kabat, David.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 272, No. 2, 30.06.1972, p. 297-304.

Research output: Contribution to journalArticle

Olsen, George D. ; Gaskill, Pauline ; Kabat, David. / Presence of hemoglobin messenger ribonucleoprotein in a reticulocyte supernatant fraction. In: BBA Section Nucleic Acids And Protein Synthesis. 1972 ; Vol. 272, No. 2. pp. 297-304.
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