Presence of hemoglobin messenger ribonucleoprotein in a reticulocyte supernatant fraction

George D. Olsen, Pauline Gaskill, David Kabat

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A study is made of globin messenger ribonucleic acid (mRNA) distribution in a rabbit reticulocyte lysate. Ribonucleic acid with the electrophoretic mobility of globin mRNA is found not only in polyribosomes but also in a ribonucleoprotein complex which sediments at 15 S. Such presumptive mRNA is not found in single ribosomes or in the subribosomal particle fraction. Further evidence that the 15-S ribonucleoprotein contains globin mRNA is obtained using a protein synthesizing extract from embryonic muscle. Addition of 15-S complex to the extract specifically stimulates incorporation of radioactive amino acids into material which cochromatographs with carrier hemoglobin and which coprecipitates with the carrier hemoglobin upon addition of anti-hemoglobin antiserum.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume272
Issue number2
DOIs
StatePublished - Jun 30 1972

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