Preparation of multivesicular liposomes

Sinil Kim; Mitchell S. Turker; Emil Y. Chi; Shifra Sela; George M. Martin

doi:10.1016/0005-2736(83)90504-7

Preparation of multivesicular liposomes

Sinil Kim, Mitchell S. Turker, Emil Y. Chi, Shifra Sela, George M. Martin

Research output: Contribution to journal › Article › peer-review

178 Scopus citations

Abstract

A novel type of liposome, named here multivesicular liposomes, was prepared by evaporation of organic solvents from chloroform-ether spherules suspended in water. Within each spherule were numerous water droplets that contained solutes to be trapped in liposomes upon solvent evaporation. Liposome preparations of different average diameters were made, varying from 29 ± 10 μm to 5.6 ± 1.7 μm. The liposomes were morphologically characterized by light microscopy and transmission electron microscopy. Materials successfully trapped within the liposomes ranged in molecular size from glucose to nucleic acids. Extremely high percentages of encapsulation (up to 89%) were achieved.

Original language	English (US)
Pages (from-to)	339-348
Number of pages	10
Journal	BBA - Biomembranes
Volume	728
Issue number	3
DOIs	https://doi.org/10.1016/0005-2736(83)90504-7
State	Published - Mar 9 1983
Externally published	Yes

Keywords

Encapsulation
Liposome preparation

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(83)90504-7

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title = "Preparation of multivesicular liposomes",

abstract = "A novel type of liposome, named here multivesicular liposomes, was prepared by evaporation of organic solvents from chloroform-ether spherules suspended in water. Within each spherule were numerous water droplets that contained solutes to be trapped in liposomes upon solvent evaporation. Liposome preparations of different average diameters were made, varying from 29 ± 10 μm to 5.6 ± 1.7 μm. The liposomes were morphologically characterized by light microscopy and transmission electron microscopy. Materials successfully trapped within the liposomes ranged in molecular size from glucose to nucleic acids. Extremely high percentages of encapsulation (up to 89%) were achieved.",

keywords = "Encapsulation, Liposome preparation",

author = "Sinil Kim and Turker, {Mitchell S.} and Chi, {Emil Y.} and Shifra Sela and Martin, {George M.}",

note = "Funding Information: We thank Mr. Chuan Teh, Mr. Daniel Teh and Ms. Marguerite Knutson for technical assistance. We also thank Dr. Dave A. Kalman of the Department of Environmental Health, University of Washington, for gas chromatography. This work was supported by Grants AG 01751, AG 00057 and GM 15253 from the NIH.",

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doi = "10.1016/0005-2736(83)90504-7",

language = "English (US)",

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TY - JOUR

T1 - Preparation of multivesicular liposomes

AU - Kim, Sinil

AU - Turker, Mitchell S.

AU - Chi, Emil Y.

AU - Sela, Shifra

AU - Martin, George M.

N1 - Funding Information: We thank Mr. Chuan Teh, Mr. Daniel Teh and Ms. Marguerite Knutson for technical assistance. We also thank Dr. Dave A. Kalman of the Department of Environmental Health, University of Washington, for gas chromatography. This work was supported by Grants AG 01751, AG 00057 and GM 15253 from the NIH.

PY - 1983/3/9

Y1 - 1983/3/9

N2 - A novel type of liposome, named here multivesicular liposomes, was prepared by evaporation of organic solvents from chloroform-ether spherules suspended in water. Within each spherule were numerous water droplets that contained solutes to be trapped in liposomes upon solvent evaporation. Liposome preparations of different average diameters were made, varying from 29 ± 10 μm to 5.6 ± 1.7 μm. The liposomes were morphologically characterized by light microscopy and transmission electron microscopy. Materials successfully trapped within the liposomes ranged in molecular size from glucose to nucleic acids. Extremely high percentages of encapsulation (up to 89%) were achieved.

AB - A novel type of liposome, named here multivesicular liposomes, was prepared by evaporation of organic solvents from chloroform-ether spherules suspended in water. Within each spherule were numerous water droplets that contained solutes to be trapped in liposomes upon solvent evaporation. Liposome preparations of different average diameters were made, varying from 29 ± 10 μm to 5.6 ± 1.7 μm. The liposomes were morphologically characterized by light microscopy and transmission electron microscopy. Materials successfully trapped within the liposomes ranged in molecular size from glucose to nucleic acids. Extremely high percentages of encapsulation (up to 89%) were achieved.

KW - Encapsulation

KW - Liposome preparation

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DO - 10.1016/0005-2736(83)90504-7

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AN - SCOPUS:0021098343

SN - 0005-2736

VL - 728

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EP - 348

JO - BBA - Biomembranes

JF - BBA - Biomembranes

IS - 3

ER -

Preparation of multivesicular liposomes

Abstract

Keywords

ASJC Scopus subject areas

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