Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay

H. Sankaran; C. W. Deveney; I. D. Goldfine; J. A. Williams

Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay

H. Sankaran, C. W. Deveney, I. D. Goldfine, J. A. Williams

Surgery

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Abstract

A modified method is described for the preparation of stable, high specific activity radioiodinated cholecystokinin (CCK) by its conjugation to ¹²⁵I-Bolton Hunter reagent (¹²⁵I-BH). Purified radioiodinated CCK (¹²⁵I-BH-CCK) stimulated amylase release from isolated rat pancreatic acini with a potency identical to that of native CCK. Further, ¹²⁵I-BH-CCK was highly immunoreactive and reacted with antisera directed toward both the NH₂- and COOH-terminal portions of the CCK molecule. Finally, ¹²⁵I-BH-CCK bound to specific receptor sites on isolated pancreatic acini.

Original language	English (US)
Pages (from-to)	9349-9351
Number of pages	3
Journal	Journal of Biological Chemistry
Volume	254
Issue number	19
State	Published - 1979

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay",

abstract = "A modified method is described for the preparation of stable, high specific activity radioiodinated cholecystokinin (CCK) by its conjugation to 125I-Bolton Hunter reagent (125I-BH). Purified radioiodinated CCK (125I-BH-CCK) stimulated amylase release from isolated rat pancreatic acini with a potency identical to that of native CCK. Further, 125I-BH-CCK was highly immunoreactive and reacted with antisera directed toward both the NH2- and COOH-terminal portions of the CCK molecule. Finally, 125I-BH-CCK bound to specific receptor sites on isolated pancreatic acini.",

author = "H. Sankaran and Deveney, {C. W.} and Goldfine, {I. D.} and Williams, {J. A.}",

year = "1979",

language = "English (US)",

volume = "254",

pages = "9349--9351",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "19",

}

TY - JOUR

T1 - Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay

AU - Sankaran, H.

AU - Deveney, C. W.

AU - Goldfine, I. D.

AU - Williams, J. A.

PY - 1979

Y1 - 1979

N2 - A modified method is described for the preparation of stable, high specific activity radioiodinated cholecystokinin (CCK) by its conjugation to 125I-Bolton Hunter reagent (125I-BH). Purified radioiodinated CCK (125I-BH-CCK) stimulated amylase release from isolated rat pancreatic acini with a potency identical to that of native CCK. Further, 125I-BH-CCK was highly immunoreactive and reacted with antisera directed toward both the NH2- and COOH-terminal portions of the CCK molecule. Finally, 125I-BH-CCK bound to specific receptor sites on isolated pancreatic acini.

AB - A modified method is described for the preparation of stable, high specific activity radioiodinated cholecystokinin (CCK) by its conjugation to 125I-Bolton Hunter reagent (125I-BH). Purified radioiodinated CCK (125I-BH-CCK) stimulated amylase release from isolated rat pancreatic acini with a potency identical to that of native CCK. Further, 125I-BH-CCK was highly immunoreactive and reacted with antisera directed toward both the NH2- and COOH-terminal portions of the CCK molecule. Finally, 125I-BH-CCK bound to specific receptor sites on isolated pancreatic acini.

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M3 - Article

C2 - 226515

AN - SCOPUS:0018712133

SN - 0021-9258

VL - 254

SP - 9349

EP - 9351

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 19

ER -

Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay

Abstract

ASJC Scopus subject areas

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