Preparation of biologically active radioiodinated cholecystokinin for radioreceptor assay and radioimmunoassay

H. Sankaran, C. W. Deveney, I. D. Goldfine, J. A. Williams

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

A modified method is described for the preparation of stable, high specific activity radioiodinated cholecystokinin (CCK) by its conjugation to 125I-Bolton Hunter reagent (125I-BH). Purified radioiodinated CCK (125I-BH-CCK) stimulated amylase release from isolated rat pancreatic acini with a potency identical to that of native CCK. Further, 125I-BH-CCK was highly immunoreactive and reacted with antisera directed toward both the NH2- and COOH-terminal portions of the CCK molecule. Finally, 125I-BH-CCK bound to specific receptor sites on isolated pancreatic acini.

Original languageEnglish (US)
Pages (from-to)9349-9351
Number of pages3
JournalJournal of Biological Chemistry
Volume254
Issue number19
StatePublished - Dec 1 1979

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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