Preparation and hybridization analysis of DNA/RNA from E. coli on microfabricated bioelectronic chips

Jing Cheng, Edward L. Sheldon, Adam Uribe, Louis O. Gerrue, John Carrino, Michael J. Heller, James P. O'Connell

Research output: Contribution to journalArticle

296 Scopus citations

Abstract

Escherichia coli were separated from a mixture containing human blood cells by means of dielectrophoresis and then subjected to electronic lysis followed by proteolytic digestion on a single microfabricated bioelectronic chip. An alternating current electric field was used to direct the bacteria to 25 microlocations above individually addressable platinum microelectrodes. The platinum electrodes were 80 μm in diameter and had center-to-center spacings of 200 μm. After the isolation, the bacteria were lysed by a series of high-voltage pulses. The lysate contained a spectrum of nucleic acids including RNA, plasmid DNA, and genomic DNA. The lysate was further examined by electronically enhanced hybridization on separate bioelectronic chips. Dielectrophoretic separation of cells followed by electronic lysis and digestion on an electronically active chip may have potential as a sample preparation process for chip-based hybridization assays in an integrated DNA/RNA analysis system.

Original languageEnglish (US)
Pages (from-to)541-546
Number of pages6
JournalNature biotechnology
Volume16
Issue number6
DOIs
StatePublished - Jun 1 1998

Keywords

  • Dielectrophoresis
  • Electronic lysis
  • Minitiaturization
  • Nanotechnology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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