Preparation and characterization of a non-viral gene vector from a polysaccharide isolated from the Chinese medicinal herb Bletilla striata

Xiang Wen Xia, Xin Li, Gan Sheng Feng, Yan Bing Zhao, Chuan Sheng Zheng

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

OBJECTIVE: To prepare the cationic product of a polysaccharide isolated from Chinese medicinal herb Bletilla striata, and to investigate the characterization and the possibility of being a non-viral gene vector. METHODS: Bletilla striata polysaccharide was prepared by ethanol precipitation followed the hot water extraction Bletilla striata stem and deproteinized with Sevag method. It was further isolated by ion-exchange chromatography on DE - 52 column and gel filtration on Sephadex G - 100 column. Cationic polysaccharide was synthesized by conjugation of spermine to oxidized polysaccharide. The ability of the caionic polysaccharide to incorporate and protect plasmid DNA was measured. RESULTS: There is no cationoid primary amine and reductive carbonyl in the polysaccharide isolated from the Chinese medicinal herb Bletilla striata. The structure of cationic Bletilla striata polysaccharide contained primary amine group was confirmed by FT-IR. The synthesized cationic product incorporated and protectd plasmid DNA. CONCLUSION: The cationic Bletilla striata polysaccharide synthesized by grafted spermine to the polysaccharide isolated from the Chinese medicinal herb Bletilla striata could incorporate and protect plasmid DNA, which would be an ideal non-viral gene vector in gene transduction.

Original languageEnglish (US)
Pages (from-to)1369-1373
Number of pages5
JournalChinese Pharmaceutical Journal
Volume43
Issue number18
StatePublished - Sep 1 2008

Keywords

  • Bletilla striata
  • Gene vector
  • Polysaccharide
  • Reductive amination

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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