Preparation and characterization of a non-viral gene vector from a polysaccharide isolated from the Chinese medicinal herb Bletilla striata

Xiang Wen Xia, Xin Li, Gan Sheng Feng, Yan Bing Zhao, Chuan Sheng Zheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

OBJECTIVE: To prepare the cationic product of a polysaccharide isolated from Chinese medicinal herb Bletilla striata, and to investigate the characterization and the possibility of being a non-viral gene vector. METHODS: Bletilla striata polysaccharide was prepared by ethanol precipitation followed the hot water extraction Bletilla striata stem and deproteinized with Sevag method. It was further isolated by ion-exchange chromatography on DE - 52 column and gel filtration on Sephadex G - 100 column. Cationic polysaccharide was synthesized by conjugation of spermine to oxidized polysaccharide. The ability of the caionic polysaccharide to incorporate and protect plasmid DNA was measured. RESULTS: There is no cationoid primary amine and reductive carbonyl in the polysaccharide isolated from the Chinese medicinal herb Bletilla striata. The structure of cationic Bletilla striata polysaccharide contained primary amine group was confirmed by FT-IR. The synthesized cationic product incorporated and protectd plasmid DNA. CONCLUSION: The cationic Bletilla striata polysaccharide synthesized by grafted spermine to the polysaccharide isolated from the Chinese medicinal herb Bletilla striata could incorporate and protect plasmid DNA, which would be an ideal non-viral gene vector in gene transduction.

Original languageEnglish (US)
Pages (from-to)1369-1373
Number of pages5
JournalChinese Pharmaceutical Journal
Volume43
Issue number18
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

Medicinal Plants
Polysaccharides
Genes
Plasmids
Spermine
Amines
DNA
Ion Exchange Chromatography
Gel Chromatography
Ethanol
Water

Keywords

  • Bletilla striata
  • Gene vector
  • Polysaccharide
  • Reductive amination

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Preparation and characterization of a non-viral gene vector from a polysaccharide isolated from the Chinese medicinal herb Bletilla striata. / Xia, Xiang Wen; Li, Xin; Feng, Gan Sheng; Zhao, Yan Bing; Zheng, Chuan Sheng.

In: Chinese Pharmaceutical Journal, Vol. 43, No. 18, 09.2008, p. 1369-1373.

Research output: Contribution to journalArticle

Xia, Xiang Wen ; Li, Xin ; Feng, Gan Sheng ; Zhao, Yan Bing ; Zheng, Chuan Sheng. / Preparation and characterization of a non-viral gene vector from a polysaccharide isolated from the Chinese medicinal herb Bletilla striata. In: Chinese Pharmaceutical Journal. 2008 ; Vol. 43, No. 18. pp. 1369-1373.
@article{109ec5f534514460915af080d0598f65,
title = "Preparation and characterization of a non-viral gene vector from a polysaccharide isolated from the Chinese medicinal herb Bletilla striata",
abstract = "OBJECTIVE: To prepare the cationic product of a polysaccharide isolated from Chinese medicinal herb Bletilla striata, and to investigate the characterization and the possibility of being a non-viral gene vector. METHODS: Bletilla striata polysaccharide was prepared by ethanol precipitation followed the hot water extraction Bletilla striata stem and deproteinized with Sevag method. It was further isolated by ion-exchange chromatography on DE - 52 column and gel filtration on Sephadex G - 100 column. Cationic polysaccharide was synthesized by conjugation of spermine to oxidized polysaccharide. The ability of the caionic polysaccharide to incorporate and protect plasmid DNA was measured. RESULTS: There is no cationoid primary amine and reductive carbonyl in the polysaccharide isolated from the Chinese medicinal herb Bletilla striata. The structure of cationic Bletilla striata polysaccharide contained primary amine group was confirmed by FT-IR. The synthesized cationic product incorporated and protectd plasmid DNA. CONCLUSION: The cationic Bletilla striata polysaccharide synthesized by grafted spermine to the polysaccharide isolated from the Chinese medicinal herb Bletilla striata could incorporate and protect plasmid DNA, which would be an ideal non-viral gene vector in gene transduction.",
keywords = "Bletilla striata, Gene vector, Polysaccharide, Reductive amination",
author = "Xia, {Xiang Wen} and Xin Li and Feng, {Gan Sheng} and Zhao, {Yan Bing} and Zheng, {Chuan Sheng}",
year = "2008",
month = "9",
language = "English (US)",
volume = "43",
pages = "1369--1373",
journal = "Chinese Pharmaceutical Journal",
issn = "1001-2494",
publisher = "Chinese Pharmaceutical Association",
number = "18",

}

TY - JOUR

T1 - Preparation and characterization of a non-viral gene vector from a polysaccharide isolated from the Chinese medicinal herb Bletilla striata

AU - Xia, Xiang Wen

AU - Li, Xin

AU - Feng, Gan Sheng

AU - Zhao, Yan Bing

AU - Zheng, Chuan Sheng

PY - 2008/9

Y1 - 2008/9

N2 - OBJECTIVE: To prepare the cationic product of a polysaccharide isolated from Chinese medicinal herb Bletilla striata, and to investigate the characterization and the possibility of being a non-viral gene vector. METHODS: Bletilla striata polysaccharide was prepared by ethanol precipitation followed the hot water extraction Bletilla striata stem and deproteinized with Sevag method. It was further isolated by ion-exchange chromatography on DE - 52 column and gel filtration on Sephadex G - 100 column. Cationic polysaccharide was synthesized by conjugation of spermine to oxidized polysaccharide. The ability of the caionic polysaccharide to incorporate and protect plasmid DNA was measured. RESULTS: There is no cationoid primary amine and reductive carbonyl in the polysaccharide isolated from the Chinese medicinal herb Bletilla striata. The structure of cationic Bletilla striata polysaccharide contained primary amine group was confirmed by FT-IR. The synthesized cationic product incorporated and protectd plasmid DNA. CONCLUSION: The cationic Bletilla striata polysaccharide synthesized by grafted spermine to the polysaccharide isolated from the Chinese medicinal herb Bletilla striata could incorporate and protect plasmid DNA, which would be an ideal non-viral gene vector in gene transduction.

AB - OBJECTIVE: To prepare the cationic product of a polysaccharide isolated from Chinese medicinal herb Bletilla striata, and to investigate the characterization and the possibility of being a non-viral gene vector. METHODS: Bletilla striata polysaccharide was prepared by ethanol precipitation followed the hot water extraction Bletilla striata stem and deproteinized with Sevag method. It was further isolated by ion-exchange chromatography on DE - 52 column and gel filtration on Sephadex G - 100 column. Cationic polysaccharide was synthesized by conjugation of spermine to oxidized polysaccharide. The ability of the caionic polysaccharide to incorporate and protect plasmid DNA was measured. RESULTS: There is no cationoid primary amine and reductive carbonyl in the polysaccharide isolated from the Chinese medicinal herb Bletilla striata. The structure of cationic Bletilla striata polysaccharide contained primary amine group was confirmed by FT-IR. The synthesized cationic product incorporated and protectd plasmid DNA. CONCLUSION: The cationic Bletilla striata polysaccharide synthesized by grafted spermine to the polysaccharide isolated from the Chinese medicinal herb Bletilla striata could incorporate and protect plasmid DNA, which would be an ideal non-viral gene vector in gene transduction.

KW - Bletilla striata

KW - Gene vector

KW - Polysaccharide

KW - Reductive amination

UR - http://www.scopus.com/inward/record.url?scp=54149102246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54149102246&partnerID=8YFLogxK

M3 - Article

VL - 43

SP - 1369

EP - 1373

JO - Chinese Pharmaceutical Journal

JF - Chinese Pharmaceutical Journal

SN - 1001-2494

IS - 18

ER -