Abstract
Pregnant Sprague-Dawley CD rats were administered 0 or 200 mg/kg of phenytoin by gavage on days 7-18 of gestation, with controls pair-fed to the phenytoin exposed dams. At birth, litter sizes were standardized to 10, balancing for sex, and were reared by their biological dams until either day 3 or 28. At each of these ages half of the litters from each group were used to determine synaptic plasma membrane order in selected brain regions (cerebellum, cortex, hippocampus) by fluorescence polarization using the probe 1,6-diphenyl-1,3,5-hexatriene (DPH). No significant differences in membrane anisotropy were noted in 3-day-old phenytoin offspring, but a reduction in anisotropy in 28-day-old phenytoin offspring hippocampal regions was observed. The effect was specific in that no changes were found in the cerebellum or cortex at this age. The magnitude of the change corresponded to an approximately 3-5°C increase in temperature and to changes produced by other known membrane disordering agents, such as ethanol. The data, together with previous findings of memory impairments in phenytoin offspring, support an association between the hippocampal membrane disordering effect and functional impairments produced by prenatal phenytoin exposure.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 283-288 |
| Number of pages | 6 |
| Journal | International Journal of Developmental Neuroscience |
| Volume | 8 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1990 |
| Externally published | Yes |
Fingerprint
Keywords
- anisotropy
- membrane fluidity
- membrane order
- phenytoin
- prenatal
- rat
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
Prenatal phenytoin exposure decreases neuronal membrane order in rat offspring hippocampus. / Vorhees, Charles V.; Rauch, Scott L.; Hitzemann, Robert.
In: International Journal of Developmental Neuroscience, Vol. 8, No. 3, 1990, p. 283-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prenatal phenytoin exposure decreases neuronal membrane order in rat offspring hippocampus
AU - Vorhees, Charles V.
AU - Rauch, Scott L.
AU - Hitzemann, Robert
PY - 1990
Y1 - 1990
N2 - Pregnant Sprague-Dawley CD rats were administered 0 or 200 mg/kg of phenytoin by gavage on days 7-18 of gestation, with controls pair-fed to the phenytoin exposed dams. At birth, litter sizes were standardized to 10, balancing for sex, and were reared by their biological dams until either day 3 or 28. At each of these ages half of the litters from each group were used to determine synaptic plasma membrane order in selected brain regions (cerebellum, cortex, hippocampus) by fluorescence polarization using the probe 1,6-diphenyl-1,3,5-hexatriene (DPH). No significant differences in membrane anisotropy were noted in 3-day-old phenytoin offspring, but a reduction in anisotropy in 28-day-old phenytoin offspring hippocampal regions was observed. The effect was specific in that no changes were found in the cerebellum or cortex at this age. The magnitude of the change corresponded to an approximately 3-5°C increase in temperature and to changes produced by other known membrane disordering agents, such as ethanol. The data, together with previous findings of memory impairments in phenytoin offspring, support an association between the hippocampal membrane disordering effect and functional impairments produced by prenatal phenytoin exposure.
AB - Pregnant Sprague-Dawley CD rats were administered 0 or 200 mg/kg of phenytoin by gavage on days 7-18 of gestation, with controls pair-fed to the phenytoin exposed dams. At birth, litter sizes were standardized to 10, balancing for sex, and were reared by their biological dams until either day 3 or 28. At each of these ages half of the litters from each group were used to determine synaptic plasma membrane order in selected brain regions (cerebellum, cortex, hippocampus) by fluorescence polarization using the probe 1,6-diphenyl-1,3,5-hexatriene (DPH). No significant differences in membrane anisotropy were noted in 3-day-old phenytoin offspring, but a reduction in anisotropy in 28-day-old phenytoin offspring hippocampal regions was observed. The effect was specific in that no changes were found in the cerebellum or cortex at this age. The magnitude of the change corresponded to an approximately 3-5°C increase in temperature and to changes produced by other known membrane disordering agents, such as ethanol. The data, together with previous findings of memory impairments in phenytoin offspring, support an association between the hippocampal membrane disordering effect and functional impairments produced by prenatal phenytoin exposure.
KW - anisotropy
KW - membrane fluidity
KW - membrane order
KW - phenytoin
KW - prenatal
KW - rat
UR - http://www.scopus.com/inward/record.url?scp=0025162431&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025162431&partnerID=8YFLogxK
U2 - 10.1016/0736-5748(90)90034-Y
DO - 10.1016/0736-5748(90)90034-Y
M3 - Article
C2 - 2386079
AN - SCOPUS:0025162431
VL - 8
SP - 283
EP - 288
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
SN - 0736-5748
IS - 3
ER -