Preliminary studies of monoclonal antibody lymphoscintigraphy in malignant melanoma

W. B. Nelp, J. F. Eary, R. F. Jones, K. E. Hellstrom, I. Hellstrom, P. L. Beaumier, Kenneth Krohn

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Lymphoscintigraphy was performed at 3 and 20 hr following subcutaneous injection of 131I anti-melanoma antibody (Fab) in 11 patients who had surgical resection of lymph nodes (neck, axilla, groint) at 24 hr for suspected metastatic melanoma. Comparable amounts of 125I nonspecific control antibody (Fab) were co-administered. Six patients had nodal metastases and three showed positive images at both time periods. Five patients had no metastases though one was image positive. Four other nondiseased inguinal node groups were image negative. A total of 28 tumored nodes and 110 normal nodes were removed, counted and histologically examined. All metastatic tumors expressed antigen against which the specific Fab was directed. The concentrations of both specific and nonspecific Fab were similar in tumored nodes and both were significantly greater than in normal nodes. Dual isotope autoradiography with video densitometric analysis of tumored nodes showed essentially identical spatial distribution of the specific and control Fab in areas containing tumor. These preliminary results suggest the increased concentration of murine immunoglobulin (Fab) retained in diseased nodes was a nonspecific phenomenon.

Original languageEnglish (US)
Pages (from-to)34-41
Number of pages8
JournalJournal of Nuclear Medicine
Volume28
Issue number1
StatePublished - 1987
Externally publishedYes

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Lymphoscintigraphy
Melanoma
Monoclonal Antibodies
Neoplasm Metastasis
Immunoglobulin Fab Fragments
Axilla
Groin
Neoplasm Antigens
Subcutaneous Injections
Autoradiography
Isotopes
Anti-Idiotypic Antibodies
Neck
Lymph Nodes
Antibodies
Neoplasms

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Nelp, W. B., Eary, J. F., Jones, R. F., Hellstrom, K. E., Hellstrom, I., Beaumier, P. L., & Krohn, K. (1987). Preliminary studies of monoclonal antibody lymphoscintigraphy in malignant melanoma. Journal of Nuclear Medicine, 28(1), 34-41.

Preliminary studies of monoclonal antibody lymphoscintigraphy in malignant melanoma. / Nelp, W. B.; Eary, J. F.; Jones, R. F.; Hellstrom, K. E.; Hellstrom, I.; Beaumier, P. L.; Krohn, Kenneth.

In: Journal of Nuclear Medicine, Vol. 28, No. 1, 1987, p. 34-41.

Research output: Contribution to journalArticle

Nelp, WB, Eary, JF, Jones, RF, Hellstrom, KE, Hellstrom, I, Beaumier, PL & Krohn, K 1987, 'Preliminary studies of monoclonal antibody lymphoscintigraphy in malignant melanoma', Journal of Nuclear Medicine, vol. 28, no. 1, pp. 34-41.
Nelp WB, Eary JF, Jones RF, Hellstrom KE, Hellstrom I, Beaumier PL et al. Preliminary studies of monoclonal antibody lymphoscintigraphy in malignant melanoma. Journal of Nuclear Medicine. 1987;28(1):34-41.
Nelp, W. B. ; Eary, J. F. ; Jones, R. F. ; Hellstrom, K. E. ; Hellstrom, I. ; Beaumier, P. L. ; Krohn, Kenneth. / Preliminary studies of monoclonal antibody lymphoscintigraphy in malignant melanoma. In: Journal of Nuclear Medicine. 1987 ; Vol. 28, No. 1. pp. 34-41.
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