Preliminary studies of monoclonal antibody lymphoscintigraphy in malignant melanoma

W. B. Nelp, J. F. Eary, R. F. Jones, K. E. Hellstrom, I. Hellstrom, P. L. Beaumier, K. A. Krohn

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25 Scopus citations

Abstract

Lymphoscintigraphy was performed at 3 and 20 hr following subcutaneous injection of 131I anti-melanoma antibody (Fab) in 11 patients who had surgical resection of lymph nodes (neck, axilla, groint) at 24 hr for suspected metastatic melanoma. Comparable amounts of 125I nonspecific control antibody (Fab) were co-administered. Six patients had nodal metastases and three showed positive images at both time periods. Five patients had no metastases though one was image positive. Four other nondiseased inguinal node groups were image negative. A total of 28 tumored nodes and 110 normal nodes were removed, counted and histologically examined. All metastatic tumors expressed antigen against which the specific Fab was directed. The concentrations of both specific and nonspecific Fab were similar in tumored nodes and both were significantly greater than in normal nodes. Dual isotope autoradiography with video densitometric analysis of tumored nodes showed essentially identical spatial distribution of the specific and control Fab in areas containing tumor. These preliminary results suggest the increased concentration of murine immunoglobulin (Fab) retained in diseased nodes was a nonspecific phenomenon.

Original languageEnglish (US)
Pages (from-to)34-41
Number of pages8
JournalJournal of Nuclear Medicine
Volume28
Issue number1
StatePublished - Apr 1 1987

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Nelp, W. B., Eary, J. F., Jones, R. F., Hellstrom, K. E., Hellstrom, I., Beaumier, P. L., & Krohn, K. A. (1987). Preliminary studies of monoclonal antibody lymphoscintigraphy in malignant melanoma. Journal of Nuclear Medicine, 28(1), 34-41.