Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system of Drosophila melanogaster

Kristofor K. Langlais, Judith A. Stewart, David Morton

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Conventional soluble guanylyl cyclases form α/β heterodimers that are activated by nitric oxide (NO). Recently, atypical members of the soluble guanylyl cyclase family have been described that include the rat β2 subunit and MsGC-β3 from Manduca sexta. Predictions from the Drosophila melanogaster genome identify three atypical guanylyl cyclase subunits: Gyc-88E (formerly CG4154), Gyc-89Da (formerly CG14885) and Gyc-89Db (formerly CG14886). Preliminary data showed that transient expression of Gyc-88E in heterologous cells generated enzyme activity in the absence of additional subunits that was slightly stimulated by the NO donor sodium nitroprusside (SNP) but not the NO donor DEA-NONOate or the NO-independent activator YC-1. Gyc-89Db was inactive when expressed alone but when co-expressed with Gyc-88E enhanced the basal and SNP-stimulated activity of Gyc-88E, suggesting that they may form heterodimers in vivo. Here, we describe the localization of Gyc-88E and Gyc-89Db and show that they are expressed in the embryonic and larval central nervous systems and are co-localized in several peripheral neurons that innervate trachea, basiconical sensilla and the sensory cones in the posterior segments of the embryo. We also show that there are two splice variants of Gyc-88E that differ by seven amino acids, although no differences in biochemical properties could be determined. We have also extended our analysis of the NO activation of Gyc-88E and Gyc-89Db, showing that several structurally unrelated NO donors activate Gyc-88E when expressed alone or when co-expressed with Gyc-89Db.

Original languageEnglish (US)
Pages (from-to)2323-2338
Number of pages16
JournalJournal of Experimental Biology
Volume207
Issue number13
DOIs
StatePublished - Jun 2004

Fingerprint

guanylate cyclase
peripheral nervous system
Nitric Oxide Donors
Peripheral Nervous System
nitric oxide
nervous system
Drosophila melanogaster
central nervous system
Nitric Oxide
Central Nervous System
Nitroprusside
Manduca
Sensilla
Guanylate Cyclase
Trachea
Embryonic Structures
sodium
Genome
Neurons
Amino Acids

Keywords

  • Central nervous system
  • cGMP
  • Chemosensory
  • Drosophila melanogaster
  • Guanylyl cyclase
  • Nitric oxide
  • NO-insensitive, sensilla
  • Peripheral nervous system

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system of Drosophila melanogaster. / Langlais, Kristofor K.; Stewart, Judith A.; Morton, David.

In: Journal of Experimental Biology, Vol. 207, No. 13, 06.2004, p. 2323-2338.

Research output: Contribution to journalArticle

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