Preliminary characterization of a soluble immunosuppressive molecule from DBA/2 spleen cells using monoclonal antibody immunoadsorbence

J. Kevin Steele, Anthea Tench Stammers, Agnes Chan, Tom Maier, Julia G. Levy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In a previous publication a monoclonal antibody (B16G) which appeared to recognize T suppressor cells and a T-suppressor factor (TsF) in the spleens of DBA/2 mice was described. B16G appears to be directed to a public specificity of DBA/2 TsF and therefore has been shown to inhibit a variety of immunological reactions. The present study involves preliminary characterization of the material with which B16G reacts. It was found that the B16G-reactive protein (putative TsF) could be absorbed and eluted specifically from a B16G immunoadsorbent column. Material eluting from the B16G column reacted with B16G in an ELISA and appeared to run as two or more bands of 40-45 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The eluted material was biologically active (i.e., suppressive) in the standard assay (mixed leukocyte reaction of DBA/2 splenocytes with B10.BR targets), and its suppressive activity was abrogated by the addition of B16G to the mixed leukocyte reaction cultures. Sephadex G-150 chromatography of the B16G-reactive material showed that under these conditions, its native molecular mass was between 80-90 kDa, indicating that it might occur as a dimer under natural conditions.

Original languageEnglish (US)
Pages (from-to)303-313
Number of pages11
JournalCellular Immunology
Volume90
Issue number2
DOIs
StatePublished - 1985
Externally publishedYes

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Immunologic Suppressor Factors
Immunosuppressive Agents
Mixed Lymphocyte Culture Test
Spleen
Monoclonal Antibodies
Immunosorbents
Inbred DBA Mouse
Sodium Dodecyl Sulfate
Chromatography
Polyacrylamide Gel Electrophoresis
Enzyme-Linked Immunosorbent Assay
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Preliminary characterization of a soluble immunosuppressive molecule from DBA/2 spleen cells using monoclonal antibody immunoadsorbence. / Steele, J. Kevin; Stammers, Anthea Tench; Chan, Agnes; Maier, Tom; Levy, Julia G.

In: Cellular Immunology, Vol. 90, No. 2, 1985, p. 303-313.

Research output: Contribution to journalArticle

Steele, J. Kevin ; Stammers, Anthea Tench ; Chan, Agnes ; Maier, Tom ; Levy, Julia G. / Preliminary characterization of a soluble immunosuppressive molecule from DBA/2 spleen cells using monoclonal antibody immunoadsorbence. In: Cellular Immunology. 1985 ; Vol. 90, No. 2. pp. 303-313.
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