Abstract
We hypothesize that ovarian hormones may improve serotonin neuron survival. We sought the effect of estradiol (E) and progesterone (P) on novel gene expression in the macaque dorsal raphe region with Affymetrix array analysis. Nine spayed rhesus macaques were treated with either placebo, E or E+P via Silastic implant for 1 month prior to euthanasia (n=3 per treatment). RNA was extracted from a small block of midbrain containing the dorsal raphe and examined on an Agilent Bioanalyzer. The RNA from each monkey was labeled and hybridized to an Affymetrix HG_U95AV Human GeneChip Array. After filtering and sorting, 25 named genes remained that were regulated by E, and 24 named genes remained that were regulated by supplemental P. These genes further sorted into functional categories that would promote neuronal plasticity, transmitter synthesis, and trafficking, as well as reduce apoptosis. The relative abundance of four pivotal genes was examined in all nine animals with quantitative RT-PCR and normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH). E±P caused a significant threefold reduction in JNK-1 (a pro-apoptosis gene, p
| Original language | English (US) |
|---|---|
| Pages (from-to) | 125-140 |
| Number of pages | 16 |
| Journal | Psychopharmacology |
| Volume | 180 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 2005 |
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Keywords
- Affymetrix array
- Apoptosis
- E2F1
- Estrogen
- GABA-A receptor
- JNK-1
- KMO
- Macaques
- Progesterone
- Serotonin
ASJC Scopus subject areas
- Pharmacology
Cite this
Preliminary array analysis reveals novel genes regulated by ovarian steroids in the monkey raphe region. / Reddy, Arubala; Bethea, Cynthia.
In: Psychopharmacology, Vol. 180, No. 1, 06.2005, p. 125-140.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preliminary array analysis reveals novel genes regulated by ovarian steroids in the monkey raphe region
AU - Reddy, Arubala
AU - Bethea, Cynthia
PY - 2005/6
Y1 - 2005/6
N2 - We hypothesize that ovarian hormones may improve serotonin neuron survival. We sought the effect of estradiol (E) and progesterone (P) on novel gene expression in the macaque dorsal raphe region with Affymetrix array analysis. Nine spayed rhesus macaques were treated with either placebo, E or E+P via Silastic implant for 1 month prior to euthanasia (n=3 per treatment). RNA was extracted from a small block of midbrain containing the dorsal raphe and examined on an Agilent Bioanalyzer. The RNA from each monkey was labeled and hybridized to an Affymetrix HG_U95AV Human GeneChip Array. After filtering and sorting, 25 named genes remained that were regulated by E, and 24 named genes remained that were regulated by supplemental P. These genes further sorted into functional categories that would promote neuronal plasticity, transmitter synthesis, and trafficking, as well as reduce apoptosis. The relative abundance of four pivotal genes was examined in all nine animals with quantitative RT-PCR and normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH). E±P caused a significant threefold reduction in JNK-1 (a pro-apoptosis gene, p
AB - We hypothesize that ovarian hormones may improve serotonin neuron survival. We sought the effect of estradiol (E) and progesterone (P) on novel gene expression in the macaque dorsal raphe region with Affymetrix array analysis. Nine spayed rhesus macaques were treated with either placebo, E or E+P via Silastic implant for 1 month prior to euthanasia (n=3 per treatment). RNA was extracted from a small block of midbrain containing the dorsal raphe and examined on an Agilent Bioanalyzer. The RNA from each monkey was labeled and hybridized to an Affymetrix HG_U95AV Human GeneChip Array. After filtering and sorting, 25 named genes remained that were regulated by E, and 24 named genes remained that were regulated by supplemental P. These genes further sorted into functional categories that would promote neuronal plasticity, transmitter synthesis, and trafficking, as well as reduce apoptosis. The relative abundance of four pivotal genes was examined in all nine animals with quantitative RT-PCR and normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH). E±P caused a significant threefold reduction in JNK-1 (a pro-apoptosis gene, p
KW - Affymetrix array
KW - Apoptosis
KW - E2F1
KW - Estrogen
KW - GABA-A receptor
KW - JNK-1
KW - KMO
KW - Macaques
KW - Progesterone
KW - Serotonin
UR - http://www.scopus.com/inward/record.url?scp=21244436362&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21244436362&partnerID=8YFLogxK
U2 - 10.1007/s00213-005-2154-1
DO - 10.1007/s00213-005-2154-1
M3 - Article
C2 - 15731897
AN - SCOPUS:21244436362
VL - 180
SP - 125
EP - 140
JO - Psychopharmacology
JF - Psychopharmacology
SN - 0033-3158
IS - 1
ER -