Preliminary array analysis reveals novel genes regulated by ovarian steroids in the monkey raphe region

Arubala Reddy, Cynthia Bethea

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    We hypothesize that ovarian hormones may improve serotonin neuron survival. We sought the effect of estradiol (E) and progesterone (P) on novel gene expression in the macaque dorsal raphe region with Affymetrix array analysis. Nine spayed rhesus macaques were treated with either placebo, E or E+P via Silastic implant for 1 month prior to euthanasia (n=3 per treatment). RNA was extracted from a small block of midbrain containing the dorsal raphe and examined on an Agilent Bioanalyzer. The RNA from each monkey was labeled and hybridized to an Affymetrix HG_U95AV Human GeneChip Array. After filtering and sorting, 25 named genes remained that were regulated by E, and 24 named genes remained that were regulated by supplemental P. These genes further sorted into functional categories that would promote neuronal plasticity, transmitter synthesis, and trafficking, as well as reduce apoptosis. The relative abundance of four pivotal genes was examined in all nine animals with quantitative RT-PCR and normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH). E±P caused a significant threefold reduction in JNK-1 (a pro-apoptosis gene, p

    Original languageEnglish (US)
    Pages (from-to)125-140
    Number of pages16
    JournalPsychopharmacology
    Volume180
    Issue number1
    DOIs
    StatePublished - Jun 2005

    Fingerprint

    Haplorhini
    Steroids
    Genes
    RNA
    Apoptosis
    Glyceraldehyde-3-Phosphate Dehydrogenases
    Neuronal Plasticity
    Euthanasia
    Macaca
    Mesencephalon
    Macaca mulatta
    Progesterone
    Estradiol
    Serotonin
    Placebos
    Hormones
    Gene Expression
    Neurons
    Polymerase Chain Reaction
    Dorsal Raphe Nucleus

    Keywords

    • Affymetrix array
    • Apoptosis
    • E2F1
    • Estrogen
    • GABA-A receptor
    • JNK-1
    • KMO
    • Macaques
    • Progesterone
    • Serotonin

    ASJC Scopus subject areas

    • Pharmacology

    Cite this

    Preliminary array analysis reveals novel genes regulated by ovarian steroids in the monkey raphe region. / Reddy, Arubala; Bethea, Cynthia.

    In: Psychopharmacology, Vol. 180, No. 1, 06.2005, p. 125-140.

    Research output: Contribution to journalArticle

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