Preferential binding of platelets to monocytes over neutrophils under flow

Kyung C. Ahn, Andy J. Jun, Parag Pawar, Sameer Jadhav, Susan Napier, Owen J.T. McCarty, Konstantinos Konstantopoulos

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

This study was undertaken to systematically investigate the binding kinetics of platelet recruitment by monocytes relative to neutrophils in bulk suspensions subjected to shear as well as the molecular requirements of leukocyte-platelet binding. Hydrodynamic shear-induced collisions augment the proportion of monocytes with adherent platelets more drastically than that of neutrophils with bound platelets. These heterotypic interactions are further potentiated by platelet activation with thrombin or to a lesser extent by monocyte stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP). Monocyte-platelet heteroaggregation increases with increasing shear rate and shear exposure time. Platelet P-selectin binding to monocyte P-selectin-glycoprotein-ligand-1 is solely responsible for maximal platelet adhesion to unstimulated monocytes in shear flow. However, the enhanced platelet binding to fMLP-treated monocytes involves a sequential two-step process, wherein P-selectin-PSGL-1 interactions are stabilized by CD18-integrin involvement. Blocking platelet αIIbβ3 or monocyte β1-integrin function had no effect. This study underscores the preferential recruitment of platelets by monocytes relative to neutrophils in shear flow, and demonstrates that the shear environment of the vasculature coupled to the state of cell activation modulates the dynamics and molecular constituents mediating monocyte-platelet adhesion.

Original languageEnglish (US)
Pages (from-to)345-355
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number1
DOIs
StatePublished - Apr 1 2005

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Keywords

  • Aggregation
  • CD18
  • Monocyte
  • Neutrophil
  • P-selectin
  • PSGL-1
  • Platelet
  • Shear
  • Thrombin
  • fMLP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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