TY - JOUR
T1 - Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis
AU - Vera-Rodriguez, Maria
AU - Chavez, Shawn L.
AU - Rubio, Carmen
AU - Reijo Pera, Renee A.
AU - Simon, Carlos
N1 - Publisher Copyright:
© 2015, Nature Publishing Group. All rights reserved.
PY - 2015/7/7
Y1 - 2015/7/7
N2 - Aneuploidies are prevalent in the human embryo and impair proper development, leading to cell cycle arrest. Recent advances in imaging and molecular and genetic analyses are postulated as promising strategies to unveil the mechanisms involved in aneuploidy generation. Here we combine time-lapse, complete chromosomal assessment and single-cell RT-qPCR to simultaneously obtain information from all cells that compose a human embryo until the approximately eight-cell stage (n=85). Our data indicate that the chromosomal status of aneuploid embryos (n=26), including those that are mosaic (n=3), correlates with significant differences in the duration of the first mitotic phase when compared with euploid embryos (n=28). Moreover, gene expression profiling suggests that a subset of genes is differentially expressed in aneuploid embryos during the first 30h of development. Thus, we propose that the chromosomal fate of an embryo is likely determined as early as the pronuclear stage and may be predicted by a 12-gene transcriptomic signature.
AB - Aneuploidies are prevalent in the human embryo and impair proper development, leading to cell cycle arrest. Recent advances in imaging and molecular and genetic analyses are postulated as promising strategies to unveil the mechanisms involved in aneuploidy generation. Here we combine time-lapse, complete chromosomal assessment and single-cell RT-qPCR to simultaneously obtain information from all cells that compose a human embryo until the approximately eight-cell stage (n=85). Our data indicate that the chromosomal status of aneuploid embryos (n=26), including those that are mosaic (n=3), correlates with significant differences in the duration of the first mitotic phase when compared with euploid embryos (n=28). Moreover, gene expression profiling suggests that a subset of genes is differentially expressed in aneuploid embryos during the first 30h of development. Thus, we propose that the chromosomal fate of an embryo is likely determined as early as the pronuclear stage and may be predicted by a 12-gene transcriptomic signature.
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U2 - 10.1038/ncomms8601
DO - 10.1038/ncomms8601
M3 - Article
C2 - 26151134
AN - SCOPUS:84936797184
SN - 2041-1723
VL - 6
JO - Nature communications
JF - Nature communications
M1 - 7601
ER -