Predicting and Promoting Human Bone Marrow MSC Chondrogenesis by Way of TGFβ Receptor Profiles: Toward Personalized Medicine

René Rothweiler, Valentina Basoli, Fabian Duttenhoefer, David Kubosch, Rainer Schmelzeisen, Brian Johnstone, Mauro Alini, Martin James Stoddart

Research output: Contribution to journalArticle

Abstract

The use of human mesenchymal stromal cells (hMSCs) for cartilage regeneration has been hampered by the inherent donor variation of primary monolayer expanded cells. Although CD markers are typically used to characterize cell populations, there is no correlation between CD marker profile and functional outcomes. Therefore, we aimed to discover novel predictive MSC chondrogenesis markers. The chondrogenic potential of primary human bone marrow MSCs (hBMSCs) over multiple passages was assessed by standard pellet culture. We confirmed that the ratio of TGFβ-RI/TGFβ-RII at the time of cell recovery from the tissue culture plastic reliably predicted chondrogenic potential. Furthermore, it is possible to prospectively characterize any human BMSC cell population as responders or non-responders with respect to chondrogenic differentiation potential. Transient increase of the ratio with siRNA knockdown of TGFβ-RII reproducibly recovered the chondrogenic differentiation ability of non-responsive MSCs. Together this offers an opportunity to produce a more functionally characterized cell population for use in autologous cartilage repair therapies.

Original languageEnglish (US)
Article number618
JournalFrontiers in Bioengineering and Biotechnology
Volume8
DOIs
StatePublished - Jun 26 2020

Keywords

  • TGF receptor
  • chondrogenic differentiation
  • mesenchymal stem cell
  • personalized medicine
  • receptor ratio

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Predicting and Promoting Human Bone Marrow MSC Chondrogenesis by Way of TGFβ Receptor Profiles: Toward Personalized Medicine'. Together they form a unique fingerprint.

  • Cite this