Precision of progesterone measurements with the use of automated immunoassay analyzers and the impact on clinical decisions for in vitro fertilization

Phillip Patton, Jeong Y. Lim, Lee R. Hickok, L. Michael Kettel, Janine M. Larson, K. Y Francis Pau

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective To compare the precision of progesterone measurements obtained with the use of immunoassays and of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Design Comparative study. Setting Academic, private practice, and in vitro fertilization (IVF) research centers. Patient(s) A total of 189 human serum samples were collected during controlled ovarian hyperstimulation and early pregnancy in women undergoing IVF. Intervention(s) Serum progesterone pools (n = 10; 0.2-4 ng/mL) were sent to four laboratory centers that used four different automated immunoassay analyzers. Progesterone was measured by immunoassay in triplicate at three separate time points (n = 9 per pool) and by LC-MS/MS in triplicate once (n = 3 per pool). Main Outcome Measure(s) Inter- and intraassay coefficients of variation (CVs) of progesterone measurements were compared for each analyzer and LC-MS/MS. Result(s) Progesterone measurements by immunoassay were highly correlated with those by LC-MS/MS. Only two analyzers had intraassay CVs

Original languageEnglish (US)
Pages (from-to)1629-1636
Number of pages8
JournalFertility and Sterility
Volume101
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Fertilization in Vitro
Immunoassay
Progesterone
Private Practice
Tandem Mass Spectrometry
Serum
Liquid Chromatography
Outcome Assessment (Health Care)
Pregnancy
Research

Keywords

  • automated analyzers
  • mass spectrometry
  • Progesterone levels in IVF

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Precision of progesterone measurements with the use of automated immunoassay analyzers and the impact on clinical decisions for in vitro fertilization. / Patton, Phillip; Lim, Jeong Y.; Hickok, Lee R.; Kettel, L. Michael; Larson, Janine M.; Pau, K. Y Francis.

In: Fertility and Sterility, Vol. 101, No. 6, 2014, p. 1629-1636.

Research output: Contribution to journalArticle

Patton, Phillip ; Lim, Jeong Y. ; Hickok, Lee R. ; Kettel, L. Michael ; Larson, Janine M. ; Pau, K. Y Francis. / Precision of progesterone measurements with the use of automated immunoassay analyzers and the impact on clinical decisions for in vitro fertilization. In: Fertility and Sterility. 2014 ; Vol. 101, No. 6. pp. 1629-1636.
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