pp60c-src expression is induced by activation of normal human T lymphocytes

Donald R. Branch, Gordon Mills

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We have re-examined whether pp60c-src, the normal cellular homologue of the transforming protein of Rous sarcoma virus, is present in human T cells. By in vitro immune-complex kinase assay or Western blotting with the anti-pp60c-src mAbs 327 or GD11, pp60c-src was found to be present in lysates of T cell lines, including the Jurkat T cell line. The 327 and GD11 mAbs have been reported to be specific for pp60c-src and not to cross-react with other src family members or other kinases. Furthermore, the size of the pp60c-src bands present on Western blotting and in vitro kinase assay were clearly different from those of p56lck or p59fyn. In addition, pp60c-src is detected in the HTLV-I-derived T cell lines S1T and C8, which lack expression of p56lck and p59fyn. RNase protection assays confirmed that pp60c-src mRNA is present in Jurkat T cells. We also found pp60c-src protein to be constitutively present in freshly isolated thymocytes. In contrast, pp60c-src was absent, or present at extremely low levels, in normal, resting peripheral blood T lymphocytes, which is in agreement with previous findings. However, after stimulation of resting T cells with the mitogenic lectin PHA or with Ab to the TCR complex, pp60c-src expression is induced in both CD4+ and CD8+ T cell subsets, with peak expression detectable 12 to 24 h after T cell activation. The levels of pp60c-src are low in all T cells except Jurkat, where levels of pp60c-src are comparable to levels found in a glioblastoma cell line (T98G). Nevertheless, significant levels of pp60c-src kinase activity are readily detectable in thymocytes and activated normal T cells as well as in T cell lines. The finding that pp60c-src is inducible following activation through the TCR suggests that pp60c-src may play a specific role in the normal T cell activation pathway.

Original languageEnglish (US)
Pages (from-to)3678-3685
Number of pages8
JournalJournal of Immunology
Volume154
Issue number8
StatePublished - Dec 1 1995
Externally publishedYes

Fingerprint

T-Lymphocytes
Cell Line
Jurkat Cells
Phosphotransferases
Thymocytes
Western Blotting
Rous sarcoma virus
Human T-lymphotropic virus 1
src-Family Kinases
T-Lymphocyte Subsets
Glioblastoma
Ribonucleases
Antigen-Antibody Complex
Lectins
Proteins
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

pp60c-src expression is induced by activation of normal human T lymphocytes. / Branch, Donald R.; Mills, Gordon.

In: Journal of Immunology, Vol. 154, No. 8, 01.12.1995, p. 3678-3685.

Research output: Contribution to journalArticle

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