Posttranscriptional regulation of beta interferon expression in erythroid Friend cells treated with gamma interferon

G. Marziali, G. Fiorucci, E. M. Coccia, Z. Percario, J. Raber, A. Battistini, G. B. Rossi, E. Affabris, G. Romeo

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Treatment of Friend erythroleukemia cells (FLC) with gamma interferon (IFN-γ) in the presence of anti-IFN-β antibodies reduces the effectiveness of the antiviral state and the induction of 2'-5'-oligoadenylate synthetase activity, indicating that the antiviral activity of IFN-γ in FLC is in part mediated by the production of IFN-β. Accordingly, IFN-γ induces a less pronounced antiviral state in FLC resistant to IFN-α/β than in wild-type cells. Moreover, while results of run-on assays indicate that both IFN-α and -β genes are constitutively transcribed in these cells, FLC treatment with IFN-γ induces only IFN-β mRNA accumulation. These results indicate that posttranscriptional mechanisms are involved in the regulation of IFN-β and -α expression by IFN-γ. The low amounts of the induced IFN-β synergize with IFN-γ in mounting the potent antiviral effect.

Original languageEnglish (US)
Pages (from-to)4130-4136
Number of pages7
JournalJournal of virology
Volume65
Issue number8
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Marziali, G., Fiorucci, G., Coccia, E. M., Percario, Z., Raber, J., Battistini, A., Rossi, G. B., Affabris, E., & Romeo, G. (1991). Posttranscriptional regulation of beta interferon expression in erythroid Friend cells treated with gamma interferon. Journal of virology, 65(8), 4130-4136. https://doi.org/10.1128/jvi.65.8.4130-4136.1991