Postsynaptic, not presynaptic NMDA receptors are required for spike-timing-dependent LTD induction

Brett C. Carter, Craig Jahr

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Long-term depression (LTD) between cortical layer 4 spiny stellate cells and layer 2/3 pyramidal cells requires the activation of NMDA receptors (NMDARs). In young rodents, this form of LTD has been repeatedly reported to require presynaptic NMDARs for its induction. Here we show that at this synapse in the somatosensory cortex of 2- to 3-week-old rats and mice, postsynaptic, not presynaptic NMDARs are required for LTD induction. First, we find no evidence for functional NMDARs in L4 neuron axons using two-photon laser scanning microscopy and two-photon glutamate uncaging. Second, we find that genetic deletion of postsynaptic, but not presynaptic NMDARs prevents LTD induction. Finally, the pharmacology of the NMDAR requirement is consistent with a nonionic signaling mechanism.

Original languageEnglish (US)
JournalNature Neuroscience
DOIs
StateAccepted/In press - Jul 11 2016

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Presynaptic Receptors
N-Methyl-D-Aspartate Receptors
Photons
Somatosensory Cortex
Pyramidal Cells
Confocal Microscopy
Synapses
Axons
Glutamic Acid
Rodentia
Pharmacology
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Postsynaptic, not presynaptic NMDA receptors are required for spike-timing-dependent LTD induction. / Carter, Brett C.; Jahr, Craig.

In: Nature Neuroscience, 11.07.2016.

Research output: Contribution to journalArticle

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