Postsynaptic, not presynaptic NMDA receptors are required for spike-timing-dependent LTD induction

Brett C. Carter, Craig E. Jahr

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Long-term depression (LTD) between cortical layer 4 spiny stellate cells and layer 2/3 pyramidal cells requires the activation of NMDA receptors (NMDARs). In young rodents, this form of LTD has been repeatedly reported to require presynaptic NMDARs for its induction. Here we show that at this synapse in the somatosensory cortex of 2- to 3-week-old rats and mice, postsynaptic, not presynaptic NMDARs are required for LTD induction. First, we find no evidence for functional NMDARs in L4 neuron axons using two-photon laser scanning microscopy and two-photon glutamate uncaging. Second, we find that genetic deletion of postsynaptic, but not presynaptic NMDARs prevents LTD induction. Finally, the pharmacology of the NMDAR requirement is consistent with a nonionic signaling mechanism.

Original languageEnglish (US)
Pages (from-to)1218-1224
Number of pages7
JournalNature Neuroscience
Volume19
Issue number9
DOIs
StatePublished - Sep 1 2016

ASJC Scopus subject areas

  • Neuroscience(all)

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