Postsynaptic glutamate transport at the climbing fiber-Purkinje cell synapse

Thomas S. Otis, Michael P. Kavanaugh, Craig Jahr

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

The role of postsynaptic, neuronal glutamate transporters in terminating signals at central excitatory synapses is not known. Stimulation of a climbing fiber input to cerebellar Purkinje cells was shown to generate an anionic current mediated by glutamate transporters. The kinetics of transporter currents were resolved by pulses of glutamate to outside-out membrane patches from Purkinje cells. Comparison of synaptic transporter currents to transporter currents expressed in Xenopus oocytes suggests that postsynaptic uptake at the climbing fiber synapse removes at least 22 percent of released glutamate. These neuronal transporter currents arise from synchronous activation of transporters that greatly outnumber activated AMPA receptors.

Original languageEnglish (US)
Pages (from-to)1515-1518
Number of pages4
JournalScience
Volume277
Issue number5331
DOIs
StatePublished - Sep 5 1997

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Amino Acid Transport System X-AG
Purkinje Cells
Synapses
Glutamic Acid
AMPA Receptors
Xenopus
Oocytes
Membranes

ASJC Scopus subject areas

  • General

Cite this

Postsynaptic glutamate transport at the climbing fiber-Purkinje cell synapse. / Otis, Thomas S.; Kavanaugh, Michael P.; Jahr, Craig.

In: Science, Vol. 277, No. 5331, 05.09.1997, p. 1515-1518.

Research output: Contribution to journalArticle

Otis, Thomas S. ; Kavanaugh, Michael P. ; Jahr, Craig. / Postsynaptic glutamate transport at the climbing fiber-Purkinje cell synapse. In: Science. 1997 ; Vol. 277, No. 5331. pp. 1515-1518.
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