Postnatal development of μ-opioid receptors in the rat caudate-putamen nucleus parallels asymmetric synapse formation

H. Wang, Verginia Cuzon Carlson, V. M. Pickel

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The μ-opioid receptor (MOR) in the caudate-putamen nucleus (CPN) appears early during prenatal development, and shows a patch-like distribution throughout the postnatal period and adulthood. In the adult rat CPN, neurons in patch compartments receive glutamatergic excitatory input mainly from the cortex through synapses onto spines, many of which express MORs. Thus, MOR expression in spines may be related to corticostriatal synaptogenesis. We used electron microscopic immunocytochemistry to determine potential age-dependent changes in the distribution pattern of MOR during postnatal synaptogenesis in the rat CPN. Immunogold-silver labeling revealed that the dendritic plasmalemmal density of MOR at postnatal day (P) 0 was significantly lower than, but after P10 was similar to, that of adult. In contrast, such age-dependent changes were not observed in axon terminals. Stereological analysis of immunoperoxidase labeling for MOR showed a good correlation in the developmental numerical densities of synapses with MOR-labeled spines and those of total asymmetric axospinous synapses, linear correlation coefficient r=0.99. Synapses with MOR-labeled dendrites, however, had a low correlation with axodendritic synapses (r=0.61), and synapses with MOR-labeled terminals showed no correlation with axospinous and axodendritic synapses (r=0.19). These results provide ultrastructural evidence that the targeting of MOR on the plasma membrane of dendrites and spines parallels the peak period of synaptogenesis during the third postnatal week in the rat CPN. Thus, the postnatal spatiotemporal expression pattern of MOR appears to match the functional maturation of corticostriatal glutamate transmission.

Original languageEnglish (US)
Pages (from-to)695-708
Number of pages14
JournalNeuroscience
Volume118
Issue number3
DOIs
StatePublished - May 25 2003
Externally publishedYes

Fingerprint

Caudate Nucleus
Putamen
Opioid Receptors
Synapses
Spine
Dendrites
Presynaptic Terminals
Silver
Glutamic Acid
Immunohistochemistry
Cell Membrane
Electrons
Neurons

Keywords

  • Development
  • Immunocytochemical electron microscopy
  • Striatum
  • Synaptogenesis
  • Ultrastructure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Postnatal development of μ-opioid receptors in the rat caudate-putamen nucleus parallels asymmetric synapse formation. / Wang, H.; Cuzon Carlson, Verginia; Pickel, V. M.

In: Neuroscience, Vol. 118, No. 3, 25.05.2003, p. 695-708.

Research output: Contribution to journalArticle

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