Postischemic attenuation of cerebral artery reactivity is increased in the presence of tissue plasminogen activator

Marilyn J. Cipolla, Nikola Lessov, Wayne Clark

Research output: Contribution to journalArticle

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Abstract

Background and Purpose - We investigated the combined effect of tissue plasminogen activator and ischemia on middle cerebral artery (MCA) reactivity to determine whether abnormal MCA function after 2 hours of ischemia was worse in arteries perfused with recombinant tissue plasminogen activator (rtPA). Methods - The intraluminal suture model of focal cerebral ischemia was used to induce 2 hours of ischemia in rats, after which occluded MCAs were removed and studied in vitro with an arteriograph system that allowed control of transmural pressure (TMP) and measurement of lumen diameter. Arteries were either nonischemic (control; n= 8), nonischemic and perfused with 400 μg/mL rtPA (rtPA; n=5), ischemic (ISC; n=6), or ischemic and perfused with 400 μg/mL rtPA (ISC-rtPA; n=6). After a 1-hour equilibration at 75 mm Hg, TMP was increased to 125 mm Hg and lumen diameter was recorded at each pressure. Reactivity to acetylcholine (ACh, 0.1 to 10.0 μmol/L) and serotonin (0.01 to 10 μmol/L) was then determined. Results - Control arteries responded myogenically to pressure and increased the amount of tone from 18.5±3.8% at 75 mm Hg to 24.8±3.0% at 125 mm Hg (P- 5mol/L ACh was 23±4% for control versus 15±2% for rtPA; 17±3% for ISC arteries (P50 (μmol/L) was 0.06±0.01 for control, 0.17±0.02 for rtPA, 0.22±0.07 for ISC, and 0.16±0.04 for ISC-rtPA (P

Original languageEnglish (US)
Pages (from-to)940-945
Number of pages6
JournalStroke
Volume31
Issue number4
StatePublished - Apr 2000

Fingerprint

Cerebral Arteries
Tissue Plasminogen Activator
Arteries
Pressure
Ischemia
Middle Cerebral Artery
Brain Ischemia
Sutures
Acetylcholine
Serotonin

Keywords

  • Arterial wall
  • Ischemia
  • Middle cerebral artery
  • Tissue plasminogen activator

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Postischemic attenuation of cerebral artery reactivity is increased in the presence of tissue plasminogen activator. / Cipolla, Marilyn J.; Lessov, Nikola; Clark, Wayne.

In: Stroke, Vol. 31, No. 4, 04.2000, p. 940-945.

Research output: Contribution to journalArticle

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abstract = "Background and Purpose - We investigated the combined effect of tissue plasminogen activator and ischemia on middle cerebral artery (MCA) reactivity to determine whether abnormal MCA function after 2 hours of ischemia was worse in arteries perfused with recombinant tissue plasminogen activator (rtPA). Methods - The intraluminal suture model of focal cerebral ischemia was used to induce 2 hours of ischemia in rats, after which occluded MCAs were removed and studied in vitro with an arteriograph system that allowed control of transmural pressure (TMP) and measurement of lumen diameter. Arteries were either nonischemic (control; n= 8), nonischemic and perfused with 400 μg/mL rtPA (rtPA; n=5), ischemic (ISC; n=6), or ischemic and perfused with 400 μg/mL rtPA (ISC-rtPA; n=6). After a 1-hour equilibration at 75 mm Hg, TMP was increased to 125 mm Hg and lumen diameter was recorded at each pressure. Reactivity to acetylcholine (ACh, 0.1 to 10.0 μmol/L) and serotonin (0.01 to 10 μmol/L) was then determined. Results - Control arteries responded myogenically to pressure and increased the amount of tone from 18.5±3.8{\%} at 75 mm Hg to 24.8±3.0{\%} at 125 mm Hg (P- 5mol/L ACh was 23±4{\%} for control versus 15±2{\%} for rtPA; 17±3{\%} for ISC arteries (P50 (μmol/L) was 0.06±0.01 for control, 0.17±0.02 for rtPA, 0.22±0.07 for ISC, and 0.16±0.04 for ISC-rtPA (P",
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AU - Lessov, Nikola

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N2 - Background and Purpose - We investigated the combined effect of tissue plasminogen activator and ischemia on middle cerebral artery (MCA) reactivity to determine whether abnormal MCA function after 2 hours of ischemia was worse in arteries perfused with recombinant tissue plasminogen activator (rtPA). Methods - The intraluminal suture model of focal cerebral ischemia was used to induce 2 hours of ischemia in rats, after which occluded MCAs were removed and studied in vitro with an arteriograph system that allowed control of transmural pressure (TMP) and measurement of lumen diameter. Arteries were either nonischemic (control; n= 8), nonischemic and perfused with 400 μg/mL rtPA (rtPA; n=5), ischemic (ISC; n=6), or ischemic and perfused with 400 μg/mL rtPA (ISC-rtPA; n=6). After a 1-hour equilibration at 75 mm Hg, TMP was increased to 125 mm Hg and lumen diameter was recorded at each pressure. Reactivity to acetylcholine (ACh, 0.1 to 10.0 μmol/L) and serotonin (0.01 to 10 μmol/L) was then determined. Results - Control arteries responded myogenically to pressure and increased the amount of tone from 18.5±3.8% at 75 mm Hg to 24.8±3.0% at 125 mm Hg (P- 5mol/L ACh was 23±4% for control versus 15±2% for rtPA; 17±3% for ISC arteries (P50 (μmol/L) was 0.06±0.01 for control, 0.17±0.02 for rtPA, 0.22±0.07 for ISC, and 0.16±0.04 for ISC-rtPA (P

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