TY - JOUR
T1 - Positional cloning of ZNF217 and NABC1
T2 - Genes amplified at 20q13.2 and overexpressed in breast carcinoma
AU - Collins, Colin
AU - Rommens, Johanna M.
AU - Kowbel, David
AU - Godfrey, Tony
AU - Tanner, Minna
AU - Hwang, Soo In
AU - Polikoff, Daniel
AU - Nonet, Genevieve
AU - Cochran, Joanne
AU - Myambo, Ken
AU - Jay, Karen E.
AU - Froula, Jeff
AU - Cloutier, Thomas
AU - Kuo, Wen Lin
AU - Yaswen, Paul
AU - Dairkee, Shanaz
AU - Giovanola, Jennifer
AU - Hutchinson, Gordon B.
AU - Isola, Jorma
AU - Kallioniemi, Olli P.
AU - Palazzolo, Mike
AU - Martin, Chris
AU - Ericsson, Cheryl
AU - Pinkel, Dan
AU - Albertson, Donna
AU - Li, Wu Bo
AU - Gray, Joe W.
PY - 1998/7/21
Y1 - 1998/7/21
N2 - We report here the molecular cloning of an ≃1-Mb region of recurrent amplification at 20q13.2 in breast cancer and other tumors and the delineation of a 260-kb common region of amplification. Analysis of the 1-Mb region produced evidence for five genes, ZNF217, ZNF218, and NABC1, PIC1L (PIC1-like), CYP24, and a pseudogene CRP (Cyclophillin Related Pseudogene). ZNF217 and NABC1 emerged as strong candidate oncogenes and were characterized in detail. NABC1 is predicted to encode a 585-aa protein of unknown function and is overexpressed in most but not all breast cancer cell lines in which it was amplified. ZNF217 is centrally located in the 260-kb common region of amplification, transcribed in multiple normal tissues, and overexpressed in all cell lines and tumors in which it is amplified and in two in which it is not. ZNF217 is predicted to encode alternately spliced, Kruppel-like transcription factors of 1,062 and 1,108 aa, each having a DNA-binding domain (eight C2H2 zinc fingers) and a proline-rich transcription activation domain.
AB - We report here the molecular cloning of an ≃1-Mb region of recurrent amplification at 20q13.2 in breast cancer and other tumors and the delineation of a 260-kb common region of amplification. Analysis of the 1-Mb region produced evidence for five genes, ZNF217, ZNF218, and NABC1, PIC1L (PIC1-like), CYP24, and a pseudogene CRP (Cyclophillin Related Pseudogene). ZNF217 and NABC1 emerged as strong candidate oncogenes and were characterized in detail. NABC1 is predicted to encode a 585-aa protein of unknown function and is overexpressed in most but not all breast cancer cell lines in which it was amplified. ZNF217 is centrally located in the 260-kb common region of amplification, transcribed in multiple normal tissues, and overexpressed in all cell lines and tumors in which it is amplified and in two in which it is not. ZNF217 is predicted to encode alternately spliced, Kruppel-like transcription factors of 1,062 and 1,108 aa, each having a DNA-binding domain (eight C2H2 zinc fingers) and a proline-rich transcription activation domain.
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U2 - 10.1073/pnas.95.15.8703
DO - 10.1073/pnas.95.15.8703
M3 - Article
C2 - 9671742
AN - SCOPUS:13144267754
SN - 0027-8424
VL - 95
SP - 8703
EP - 8708
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 15
ER -