Positional cloning of a temperature-sensitive mutant emmental reveals a role for Sly1 during cell proliferation in zebrafish fin regeneration

Alex Nechiporuk, Kenneth D. Poss, Stephen L. Johnson, Mark T. Keating

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Here, we used classical genetics in zebrafish to identify temperature-sensitive mutants in caudal fin regeneration. Gross morphological, histological, and molecular analyses revealed that one of these strains, emmental (emm), failed to form a functional regeneration blastema. Inhibition of emm function by heat treatment during regenerative outgrowth rapidly blocked regeneration. This block was associated with reduced proliferation in the proximal blastema and expansion of the nonproliferative distal blastemal zone. Positional cloning revealed that the emm phenotype is caused by a mutation in the orthologue of yeast sly1, a gene product involved in protein trafficking. sly1 is upregulated in the newly formed blastema as well as during regenerative outgrowth. Thus, sly1 is essential for blastemal organization and proliferation during two stages of fin regeneration.

Original languageEnglish (US)
Pages (from-to)291-306
Number of pages16
JournalDevelopmental Biology
Volume258
Issue number2
DOIs
StatePublished - Jun 15 2003
Externally publishedYes

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Zebrafish
Organism Cloning
Regeneration
Cell Proliferation
Temperature
Protein Transport
Hot Temperature
Yeasts
Phenotype
Mutation
Genes
Therapeutics

Keywords

  • Blastema
  • emmental
  • Fin
  • Genetics
  • Regeneration
  • Sly1
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Positional cloning of a temperature-sensitive mutant emmental reveals a role for Sly1 during cell proliferation in zebrafish fin regeneration. / Nechiporuk, Alex; Poss, Kenneth D.; Johnson, Stephen L.; Keating, Mark T.

In: Developmental Biology, Vol. 258, No. 2, 15.06.2003, p. 291-306.

Research output: Contribution to journalArticle

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