Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T

Nandhinee Radha Shanmugam, Anjan Panneer Selvam, Thomas W. Barrett, Steven C. Kazmierczak, Milin Nilesh Rana, Shalini Prasad

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Aim: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. Materials & methods: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy. Results: The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml. Conclusion: This novel technology has promising preliminary results for rapid and sensitive detection of cTnT. Quantification of biomarkers is essential for disease diagnosis and management. Current laboratory-based analytical methods are labor-intensive and rely mostly on use of labels for detection. A simple, point-of-care method based on a label-free technique offers robust real-time measurements for detection of biomarkers. This study intended to develop a nanoporous electrical biosensor to measure the cardiac biomarker cardiac Troponin-T. A nanotechnology approach towards sensor design improves detection sensitivity. The sensor performance for detection of cardiac Troponin-T demonstrates the potential for adoption in a clinical setting. However, further validation experiments are required prior to implementation.

Original languageEnglish (US)
Article numberFSO24
JournalFuture Science OA
Volume1
Issue number3
DOIs
StatePublished - Nov 2015

Keywords

  • Troponin-T
  • electrical double layer
  • impedance spectroscopy
  • nanoconfinement
  • nanoporous

ASJC Scopus subject areas

  • Biotechnology

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    Shanmugam, N. R., Selvam, A. P., Barrett, T. W., Kazmierczak, S. C., Rana, M. N., & Prasad, S. (2015). Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T. Future Science OA, 1(3), [FSO24]. https://doi.org/10.4155/fso.15.22