Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T

Nandhinee Radha Shanmugam, Anjan Panneer Selvam, Thomas W. Barrett, Steven (Steve) Kazmierczak, Milin Nilesh Rana, Shalini Prasad

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aim: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. Materials & methods: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy. Results: The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml. Conclusion: This novel technology has promising preliminary results for rapid and sensitive detection of cTnT. Quantification of biomarkers is essential for disease diagnosis and management. Current laboratory-based analytical methods are labor-intensive and rely mostly on use of labels for detection. A simple, point-of-care method based on a label-free technique offers robust real-time measurements for detection of biomarkers. This study intended to develop a nanoporous electrical biosensor to measure the cardiac biomarker cardiac Troponin-T. A nanotechnology approach towards sensor design improves detection sensitivity. The sensor performance for detection of cardiac Troponin-T demonstrates the potential for adoption in a clinical setting. However, further validation experiments are required prior to implementation.

Original languageEnglish (US)
Article numberFSO24
JournalFuture Science OA
Volume1
Issue number3
DOIs
StatePublished - Nov 1 2015

Fingerprint

Troponin T
Biosensing Techniques
Biomarkers
Electric Impedance
Point-of-Care Systems
Dielectric Spectroscopy
Nanotechnology
Microelectrodes
Disease Management
Limit of Detection
Technology
Antigens
Membranes

Keywords

  • electrical double layer
  • impedance spectroscopy
  • nanoconfinement
  • nanoporous
  • Troponin-T

ASJC Scopus subject areas

  • Biotechnology

Cite this

Shanmugam, N. R., Selvam, A. P., Barrett, T. W., Kazmierczak, S. S., Rana, M. N., & Prasad, S. (2015). Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T. Future Science OA, 1(3), [FSO24]. https://doi.org/10.4155/fso.15.22

Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T. / Shanmugam, Nandhinee Radha; Selvam, Anjan Panneer; Barrett, Thomas W.; Kazmierczak, Steven (Steve); Rana, Milin Nilesh; Prasad, Shalini.

In: Future Science OA, Vol. 1, No. 3, FSO24, 01.11.2015.

Research output: Contribution to journalArticle

Shanmugam, Nandhinee Radha ; Selvam, Anjan Panneer ; Barrett, Thomas W. ; Kazmierczak, Steven (Steve) ; Rana, Milin Nilesh ; Prasad, Shalini. / Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T. In: Future Science OA. 2015 ; Vol. 1, No. 3.
@article{1ba3574d04444169b17cc3376392c61a,
title = "Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T",
abstract = "Aim: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. Materials & methods: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy. Results: The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml. Conclusion: This novel technology has promising preliminary results for rapid and sensitive detection of cTnT. Quantification of biomarkers is essential for disease diagnosis and management. Current laboratory-based analytical methods are labor-intensive and rely mostly on use of labels for detection. A simple, point-of-care method based on a label-free technique offers robust real-time measurements for detection of biomarkers. This study intended to develop a nanoporous electrical biosensor to measure the cardiac biomarker cardiac Troponin-T. A nanotechnology approach towards sensor design improves detection sensitivity. The sensor performance for detection of cardiac Troponin-T demonstrates the potential for adoption in a clinical setting. However, further validation experiments are required prior to implementation.",
keywords = "electrical double layer, impedance spectroscopy, nanoconfinement, nanoporous, Troponin-T",
author = "Shanmugam, {Nandhinee Radha} and Selvam, {Anjan Panneer} and Barrett, {Thomas W.} and Kazmierczak, {Steven (Steve)} and Rana, {Milin Nilesh} and Shalini Prasad",
year = "2015",
month = "11",
day = "1",
doi = "10.4155/fso.15.22",
language = "English (US)",
volume = "1",
journal = "Future Science OA",
issn = "2056-5623",
publisher = "Future Medicine Ltd.",
number = "3",

}

TY - JOUR

T1 - Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T

AU - Shanmugam, Nandhinee Radha

AU - Selvam, Anjan Panneer

AU - Barrett, Thomas W.

AU - Kazmierczak, Steven (Steve)

AU - Rana, Milin Nilesh

AU - Prasad, Shalini

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Aim: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. Materials & methods: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy. Results: The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml. Conclusion: This novel technology has promising preliminary results for rapid and sensitive detection of cTnT. Quantification of biomarkers is essential for disease diagnosis and management. Current laboratory-based analytical methods are labor-intensive and rely mostly on use of labels for detection. A simple, point-of-care method based on a label-free technique offers robust real-time measurements for detection of biomarkers. This study intended to develop a nanoporous electrical biosensor to measure the cardiac biomarker cardiac Troponin-T. A nanotechnology approach towards sensor design improves detection sensitivity. The sensor performance for detection of cardiac Troponin-T demonstrates the potential for adoption in a clinical setting. However, further validation experiments are required prior to implementation.

AB - Aim: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. Materials & methods: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy. Results: The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml. Conclusion: This novel technology has promising preliminary results for rapid and sensitive detection of cTnT. Quantification of biomarkers is essential for disease diagnosis and management. Current laboratory-based analytical methods are labor-intensive and rely mostly on use of labels for detection. A simple, point-of-care method based on a label-free technique offers robust real-time measurements for detection of biomarkers. This study intended to develop a nanoporous electrical biosensor to measure the cardiac biomarker cardiac Troponin-T. A nanotechnology approach towards sensor design improves detection sensitivity. The sensor performance for detection of cardiac Troponin-T demonstrates the potential for adoption in a clinical setting. However, further validation experiments are required prior to implementation.

KW - electrical double layer

KW - impedance spectroscopy

KW - nanoconfinement

KW - nanoporous

KW - Troponin-T

UR - http://www.scopus.com/inward/record.url?scp=85034252449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034252449&partnerID=8YFLogxK

U2 - 10.4155/fso.15.22

DO - 10.4155/fso.15.22

M3 - Article

AN - SCOPUS:85034252449

VL - 1

JO - Future Science OA

JF - Future Science OA

SN - 2056-5623

IS - 3

M1 - FSO24

ER -