Polyphosphate nanoparticles on the platelet surface trigger contact system activation

Johan J F Verhoef, Arjan D. Barendrecht, Katrin F. Nickel, Kim Dijkxhoorn, Ellinor Kenne, Linda Labberton, Owen McCarty, Raymond Schiffelers, Harry F. Heijnen, Antoni P. Hendrickx, Huub Schellekens, Marcel H. Fens, Steven De Maat, Thomas Renné, Coen Maas

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Polyphosphate is an inorganic polymer that can potentiate several interactions in the blood coagulation system. Blood platelets contain polyphosphate, and the secretion of platelet-derived polyphosphate has been associated with increased thrombus formation and activation of coagulation factor XII. However, the small polymer size of secreted platelet polyphosphate limits its capacity to activate factor XII in vitro. Thus, the mechanismby which platelet polyphosphate contributes to thrombus formation remains unclear. Using live-cell imaging, confocal and electron microscopy, we show that activated platelets retain polyphosphate on their cell surface. The apparent polymer size of membrane-associated polyphosphate largely exceeds that of secreted polyphosphate. Ultracentrifugation fractionation experiments revealed that membrane-associated platelet polyphosphate is condensed into insoluble spherical nanoparticles with divalent metal ions. In contrast to soluble polyphosphate, membrane-associated polyphosphate nanoparticles potently activate factor XII. Our findings identify membrane-associated polyphosphate in a nanoparticle state on the surface of activated platelets. We propose that these polyphosphate nanoparticles mechanistically link the procoagulant activity of platelets with the activation of coagulation factor XII.

Original languageEnglish (US)
Pages (from-to)1707-1717
Number of pages11
JournalBlood
Volume129
Issue number12
DOIs
StatePublished - Mar 23 2017

Fingerprint

Polyphosphates
Platelets
Nanoparticles
Blood Platelets
Chemical activation
Factor XII
Membranes
Polymers
Thrombosis
Blood
Inorganic polymers
Confocal microscopy
Ultracentrifugation
Platelet Activation
Blood Coagulation
Fractionation
Coagulation
Confocal Microscopy
Electron microscopy
Metal ions

ASJC Scopus subject areas

  • Immunology
  • Biochemistry
  • Hematology
  • Cell Biology

Cite this

Verhoef, J. J. F., Barendrecht, A. D., Nickel, K. F., Dijkxhoorn, K., Kenne, E., Labberton, L., ... Maas, C. (2017). Polyphosphate nanoparticles on the platelet surface trigger contact system activation. Blood, 129(12), 1707-1717. https://doi.org/10.1182/blood-2016-08-734988

Polyphosphate nanoparticles on the platelet surface trigger contact system activation. / Verhoef, Johan J F; Barendrecht, Arjan D.; Nickel, Katrin F.; Dijkxhoorn, Kim; Kenne, Ellinor; Labberton, Linda; McCarty, Owen; Schiffelers, Raymond; Heijnen, Harry F.; Hendrickx, Antoni P.; Schellekens, Huub; Fens, Marcel H.; De Maat, Steven; Renné, Thomas; Maas, Coen.

In: Blood, Vol. 129, No. 12, 23.03.2017, p. 1707-1717.

Research output: Contribution to journalArticle

Verhoef, JJF, Barendrecht, AD, Nickel, KF, Dijkxhoorn, K, Kenne, E, Labberton, L, McCarty, O, Schiffelers, R, Heijnen, HF, Hendrickx, AP, Schellekens, H, Fens, MH, De Maat, S, Renné, T & Maas, C 2017, 'Polyphosphate nanoparticles on the platelet surface trigger contact system activation', Blood, vol. 129, no. 12, pp. 1707-1717. https://doi.org/10.1182/blood-2016-08-734988
Verhoef JJF, Barendrecht AD, Nickel KF, Dijkxhoorn K, Kenne E, Labberton L et al. Polyphosphate nanoparticles on the platelet surface trigger contact system activation. Blood. 2017 Mar 23;129(12):1707-1717. https://doi.org/10.1182/blood-2016-08-734988
Verhoef, Johan J F ; Barendrecht, Arjan D. ; Nickel, Katrin F. ; Dijkxhoorn, Kim ; Kenne, Ellinor ; Labberton, Linda ; McCarty, Owen ; Schiffelers, Raymond ; Heijnen, Harry F. ; Hendrickx, Antoni P. ; Schellekens, Huub ; Fens, Marcel H. ; De Maat, Steven ; Renné, Thomas ; Maas, Coen. / Polyphosphate nanoparticles on the platelet surface trigger contact system activation. In: Blood. 2017 ; Vol. 129, No. 12. pp. 1707-1717.
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AU - Heijnen, Harry F.

AU - Hendrickx, Antoni P.

AU - Schellekens, Huub

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AU - De Maat, Steven

AU - Renné, Thomas

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N2 - Polyphosphate is an inorganic polymer that can potentiate several interactions in the blood coagulation system. Blood platelets contain polyphosphate, and the secretion of platelet-derived polyphosphate has been associated with increased thrombus formation and activation of coagulation factor XII. However, the small polymer size of secreted platelet polyphosphate limits its capacity to activate factor XII in vitro. Thus, the mechanismby which platelet polyphosphate contributes to thrombus formation remains unclear. Using live-cell imaging, confocal and electron microscopy, we show that activated platelets retain polyphosphate on their cell surface. The apparent polymer size of membrane-associated polyphosphate largely exceeds that of secreted polyphosphate. Ultracentrifugation fractionation experiments revealed that membrane-associated platelet polyphosphate is condensed into insoluble spherical nanoparticles with divalent metal ions. In contrast to soluble polyphosphate, membrane-associated polyphosphate nanoparticles potently activate factor XII. Our findings identify membrane-associated polyphosphate in a nanoparticle state on the surface of activated platelets. We propose that these polyphosphate nanoparticles mechanistically link the procoagulant activity of platelets with the activation of coagulation factor XII.

AB - Polyphosphate is an inorganic polymer that can potentiate several interactions in the blood coagulation system. Blood platelets contain polyphosphate, and the secretion of platelet-derived polyphosphate has been associated with increased thrombus formation and activation of coagulation factor XII. However, the small polymer size of secreted platelet polyphosphate limits its capacity to activate factor XII in vitro. Thus, the mechanismby which platelet polyphosphate contributes to thrombus formation remains unclear. Using live-cell imaging, confocal and electron microscopy, we show that activated platelets retain polyphosphate on their cell surface. The apparent polymer size of membrane-associated polyphosphate largely exceeds that of secreted polyphosphate. Ultracentrifugation fractionation experiments revealed that membrane-associated platelet polyphosphate is condensed into insoluble spherical nanoparticles with divalent metal ions. In contrast to soluble polyphosphate, membrane-associated polyphosphate nanoparticles potently activate factor XII. Our findings identify membrane-associated polyphosphate in a nanoparticle state on the surface of activated platelets. We propose that these polyphosphate nanoparticles mechanistically link the procoagulant activity of platelets with the activation of coagulation factor XII.

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