Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes

P. Rotwein; R. Chyn; J. Chirgwin; B. Cordell; H. M. Goodman; M. A. Permut

doi:10.1126/science.6267694

Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes

P. Rotwein, R. Chyn, J. Chirgwin, B. Cordell, H. M. Goodman, M. A. Permut

Research output: Contribution to journal › Article › peer-review

Abstract

The arrangement of the human insulin gene in DNA from 87 individuals was analyzed by the Southern blot hybridization technique with a cloned genomic human insulin probe. Insertions of 1.5 to 3.4 kilobase pairs in the 5'-flanking region of the gene were found in DNA from 38 individuals. These insertions occurred within 1.3 kilobase pairs of the transcription initiation site. In contrast, no insertions were observed in the region 3' to the coding sequence. The prevalence of these insertions in type 2 diabetes was significantly greater than in the other groups (P

Original language	English (US)
Pages (from-to)	1117-1120
Number of pages	4
Journal	Science
Volume	213
Issue number	4512
DOIs	https://doi.org/10.1126/science.6267694
State	Published - 1981
Externally published	Yes

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abstract = "The arrangement of the human insulin gene in DNA from 87 individuals was analyzed by the Southern blot hybridization technique with a cloned genomic human insulin probe. Insertions of 1.5 to 3.4 kilobase pairs in the 5'-flanking region of the gene were found in DNA from 38 individuals. These insertions occurred within 1.3 kilobase pairs of the transcription initiation site. In contrast, no insertions were observed in the region 3' to the coding sequence. The prevalence of these insertions in type 2 diabetes was significantly greater than in the other groups (P",

author = "P. Rotwein and R. Chyn and J. Chirgwin and B. Cordell and Goodman, {H. M.} and Permut, {M. A.}",

year = "1981",

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AU - Rotwein, P.

AU - Chyn, R.

AU - Chirgwin, J.

AU - Cordell, B.

AU - Goodman, H. M.

AU - Permut, M. A.

PY - 1981

Y1 - 1981

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AB - The arrangement of the human insulin gene in DNA from 87 individuals was analyzed by the Southern blot hybridization technique with a cloned genomic human insulin probe. Insertions of 1.5 to 3.4 kilobase pairs in the 5'-flanking region of the gene were found in DNA from 38 individuals. These insertions occurred within 1.3 kilobase pairs of the transcription initiation site. In contrast, no insertions were observed in the region 3' to the coding sequence. The prevalence of these insertions in type 2 diabetes was significantly greater than in the other groups (P

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JO - Science

JF - Science

IS - 4512

ER -

Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes

Abstract

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