Abstract
We studied the effect of intravenously administered polyethylene glycol-conjugated superoxide dismutase (8,000 units/kg) on brain su peroxide dismutase activity in 441-2-week-old piglets in the absence and presence of global cerebral ischemia and reperfusion. Four groups (n=6 each) of piglets not exposed to ischemia were studied. Enzyme administration increased plasma superoxide dismutase activity from <5 to 142±8 units/ml (mean±SEM) without increasing brain activity (e.g., activities in the caudate were 7.9±0.5 and 8.1 ±0.4 units/mg protein) for up to 2 hours following administration. Four additional groups (n=5 each) of piglets were given either enzyme or polyethylene glycol 5 minutes prior to 10 minutes of global cerebral ischemia induced by aortic cross-clamping followed by either 5 or 45 minutes of reperfusion. Enzyme administration increased plasma superoxide dismutase activity from <5 to 144±5 units/ml but failed to increase brain activity even after 45 minutes of reperfusion (e.g., activities in the caudate were 8.5±03 and 8.6±0.6 units/mg protein). We conclude that intravenous polyethylene glycol-conjugated superoxide dismutase does not increase superoxide dismutase activity in the brain despite global ischemia and reperfusion.
Original language | English (US) |
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Pages (from-to) | 655-659 |
Number of pages | 5 |
Journal | Stroke |
Volume | 22 |
Issue number | 5 |
DOIs | |
State | Published - May 1991 |
Externally published | Yes |
Keywords
- Free radicals
- Pigs
- Reperfusion
- Scerebral ischemia
ASJC Scopus subject areas
- Clinical Neurology
- Cardiology and Cardiovascular Medicine
- Advanced and Specialized Nursing