Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity

D. N. Gilbert, C. A. Wood, S. J. Kohlhepp, P. W. Kohnen, Donald Houghton, H. C. Finkbeiner, J. Lindsley, W. M. Bennett

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Abstract

The influence of the polyamino acid polyaspartic acid (PAA) on experimental aminoglycoside nephrotoxicity was determined. PAA prevented all measured functional and pathologic evidence of gentamicin nephrotoxicity for ≤ 27 d of study. All the animals given PAA, either alone or with gentamicin, developed prominent cytoplasmic vacuoles in the cells of the renal proximal convoluted tubules; the vacuoles in rats given just PAA differed from those observed in rats given PAA plus gentamicin. Rats given PAA plus gentamicin accumulated roughly 10 times more renal aminoglycoside as did rats given gentamicin alone. Immunohistochemical localization studies confirmed the presence of increased amounts of gentamicin in the cytoplasm of the tubular cells of animals given gentamicin plus PAA. PAA did not alter the in vitro antimicrobial activity of gentamicin versus Escherichia coli or Pseudomonas aeruginosa. These studies demonstrate the ability of PAA to prevent experimental gentamicin nephrotoxicity.

Original languageEnglish (US)
Pages (from-to)945-953
Number of pages9
JournalJournal of Infectious Diseases
Volume159
Issue number5
StatePublished - 1989

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Aminoglycosides
Gentamicins
Vacuoles
polyaspartate
Proximal Kidney Tubule
Pseudomonas aeruginosa
Cytoplasm
Escherichia coli
Kidney
Acids

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Gilbert, D. N., Wood, C. A., Kohlhepp, S. J., Kohnen, P. W., Houghton, D., Finkbeiner, H. C., ... Bennett, W. M. (1989). Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity. Journal of Infectious Diseases, 159(5), 945-953.

Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity. / Gilbert, D. N.; Wood, C. A.; Kohlhepp, S. J.; Kohnen, P. W.; Houghton, Donald; Finkbeiner, H. C.; Lindsley, J.; Bennett, W. M.

In: Journal of Infectious Diseases, Vol. 159, No. 5, 1989, p. 945-953.

Research output: Contribution to journalArticle

Gilbert, DN, Wood, CA, Kohlhepp, SJ, Kohnen, PW, Houghton, D, Finkbeiner, HC, Lindsley, J & Bennett, WM 1989, 'Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity', Journal of Infectious Diseases, vol. 159, no. 5, pp. 945-953.
Gilbert DN, Wood CA, Kohlhepp SJ, Kohnen PW, Houghton D, Finkbeiner HC et al. Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity. Journal of Infectious Diseases. 1989;159(5):945-953.
Gilbert, D. N. ; Wood, C. A. ; Kohlhepp, S. J. ; Kohnen, P. W. ; Houghton, Donald ; Finkbeiner, H. C. ; Lindsley, J. ; Bennett, W. M. / Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity. In: Journal of Infectious Diseases. 1989 ; Vol. 159, No. 5. pp. 945-953.
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