Polyamine distribution in the rat intestinal mucosa

M. Hosomi, Stephen Smith, G. M. Murphy, R. H. Dowling

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

As the first step in a study of mucosal polyamine metabolism during intestinal adaptation, we have measured mucosal polyamine concentrations at different sites along the normal rat intestine. Putrescine, spermidine, spermine and cadaverine were measured by spectrofluorometric analysis after thin-layer chromatography of their dansylated derivatives. Spermidine was present in the largest amounts at each of the sampling sites. The ratio of the concentration of spermidine to that of spermine paralleled the established pattern of cellular proliferation in the normal intestine as did the putrescine concentration (nmol per 10 cm) which decreased from duodenum to colon. These results provide the essential background to an assessment of the role of polyamines in the intestinal adaptive response.

Original languageEnglish (US)
Pages (from-to)267-275
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume375
Issue numberC
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Spermidine
Polyamines
Intestinal Mucosa
Rats
Putrescine
Spermine
Intestines
Cadaverine
Thin layer chromatography
Thin Layer Chromatography
Duodenum
Metabolism
Colon
Cell Proliferation
Sampling
Derivatives
Mucous Membrane

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Polyamine distribution in the rat intestinal mucosa. / Hosomi, M.; Smith, Stephen; Murphy, G. M.; Dowling, R. H.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 375, No. C, 1986, p. 267-275.

Research output: Contribution to journalArticle

Hosomi, M. ; Smith, Stephen ; Murphy, G. M. ; Dowling, R. H. / Polyamine distribution in the rat intestinal mucosa. In: Journal of Chromatography B: Biomedical Sciences and Applications. 1986 ; Vol. 375, No. C. pp. 267-275.
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