Polyamine distribution in the rat intestinal mucosa

M. Hosomi, S. M. Smith, G. M. Murphy, R. H. Dowling

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

As the first step in a study of mucosal polyamine metabolism during intestinal adaptation, we have measured mucosal polyamine concentrations at different sites along the normal rat intestine. Putrescine, spermidine, spermine and cadaverine were measured by spectrofluorometric analysis after thin-layer chromatography of their dansylated derivatives. Spermidine was present in the largest amounts at each of the sampling sites. The ratio of the concentration of spermidine to that of spermine paralleled the established pattern of cellular proliferation in the normal intestine as did the putrescine concentration (nmol per 10 cm) which decreased from duodenum to colon. These results provide the essential background to an assessment of the role of polyamines in the intestinal adaptive response.

Original languageEnglish (US)
Pages (from-to)267-275
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume375
Issue numberC
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Chemistry(all)

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