Polyamine and pentamidine metabolism in African trypanosomes

Bradley J. Berger, Nicola S. Carter, Alan H. Fairlamb

Research output: Contribution to journalReview article

23 Scopus citations

Abstract

A pentamidine-resistant line of bloodstream Trypanosoma brucei brucei (S427/118) has been developed by stepwise selection in axenic culture in vitro. After 57 days of selection, the resistant line (S427/118/PR32) was able to grow normally in 32 ng/ml (54 pM) pentamidine with an IC50 value of 105 ng/ml (177 pM), which is 26-times higher than that of the parental strain. Post-mitochondrial supernatant extracts of both strains were unable to metabolize [3H]pentamidine, whereas under identical conditions rat liver microsomes were able to convert >5% of the drug to hydroxylation products. Thus metabolic conversion of pentamidine does not appear to be involved in either the mode of action of or resistance to pentamidine. Pentamidine-sensitive trypanosomes exposed for 4 h in vivo to therapeutic doses of pentamidine (4 mg/kg) did not show any significant changes in either polyamine-, thiol- or S-adenosylmethionine metabolites, indicating that inhibition of S-adenosylmethionine decarboxylase is not involved in the trypanocidal action of the drug. However, a marked increase in basic amino acid content was noted. In particular, lysine content was increased 13-fold following exposure to pentamidine.

Original languageEnglish (US)
Pages (from-to)215-224
Number of pages10
JournalActa Tropica
Volume54
Issue number3-4
DOIs
StatePublished - Sep 1993

Keywords

  • Drug metabolism
  • Drug resistance
  • Pentamidine
  • Polyamine metabolism
  • Trypanosoma brucei
  • Trypanosomiasis

ASJC Scopus subject areas

  • Parasitology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

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  • Cite this

    Berger, B. J., Carter, N. S., & Fairlamb, A. H. (1993). Polyamine and pentamidine metabolism in African trypanosomes. Acta Tropica, 54(3-4), 215-224. https://doi.org/10.1016/0001-706X(93)90094-R