TY - JOUR
T1 - Polarized light imaging specifies the anisotropy of light scattering in the superficial layer of a tissue
AU - Jacques, Steven L.
AU - Roussel, Stéphane
AU - Samatham, Ravikant
N1 - Publisher Copyright:
© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - This report describes how optical images acquired using linearly polarized light can specify the anisotropy of scattering (g) and the ratio of reduced scattering [μs′=μs(1-g)] to absorption (μa), i.e., N′=μs′/μa. A camera acquired copolarized (HH) and crosspolarized (HV) reflectance images of a tissue (skin), which yielded images based on the intensity (I=HH+HV) and difference (Q=HH-HV) of reflectance images. Monte Carlo simulations generated an analysis grid (or lookup table), which mapped Q and I into a grid of g versus N′, i.e., g(Q,I) and N′(Q,I). The anisotropy g is interesting because it is sensitive to the submicrometer structure of biological tissues. Hence, polarized light imaging can monitor shifts in the submicrometer (50 to 1000 nm) structure of tissues. The Q values for forearm skin on two subjects (one Caucasian, one pigmented) were in the range of 0.046±0.007 (24), which is the mean±SD for 24 measurements on 8 skin sites×3 visible wavelengths, 470, 524, and 625 nm, which indicated g values of 0.67±0.07 (24).
AB - This report describes how optical images acquired using linearly polarized light can specify the anisotropy of scattering (g) and the ratio of reduced scattering [μs′=μs(1-g)] to absorption (μa), i.e., N′=μs′/μa. A camera acquired copolarized (HH) and crosspolarized (HV) reflectance images of a tissue (skin), which yielded images based on the intensity (I=HH+HV) and difference (Q=HH-HV) of reflectance images. Monte Carlo simulations generated an analysis grid (or lookup table), which mapped Q and I into a grid of g versus N′, i.e., g(Q,I) and N′(Q,I). The anisotropy g is interesting because it is sensitive to the submicrometer structure of biological tissues. Hence, polarized light imaging can monitor shifts in the submicrometer (50 to 1000 nm) structure of tissues. The Q values for forearm skin on two subjects (one Caucasian, one pigmented) were in the range of 0.046±0.007 (24), which is the mean±SD for 24 measurements on 8 skin sites×3 visible wavelengths, 470, 524, and 625 nm, which indicated g values of 0.67±0.07 (24).
KW - Anisotropy
KW - Biological tissues
KW - Biomedical optics
KW - Polarized light
KW - Scattering
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U2 - 10.1117/1.JBO.21.7.071115
DO - 10.1117/1.JBO.21.7.071115
M3 - Article
C2 - 27165546
AN - SCOPUS:84977522963
SN - 1083-3668
VL - 21
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 7
M1 - 071115
ER -