Pleiotropic effects of cryptoChromes 1 and 2 on free-running and light-entrained murine circadian rhythms

Russell N. Van Gelder, Therese M. Gibler, Daniel Tu, Kris Embry, Christopher P. Selby, Carol L. Thompson, Aziz Sancar

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Cryptochromes function in both light entrainment of circadian rhythms and in a peripheral circadian clock mechanism in Drosophila. Mice have two closely related cryptochrome genes (mCry1 and mCry2). To further understand the roles of mammalian cryptochromes, we bred mice to carry all possible combinations of wild-type and cryptochrome knockout alleles, and tested these mice for free-running and entrained circadian rhythmicity. We find that a single wild-type copy of mCry1, but not mCry2, is sufficient for free running circadian rhythmicity; however, these mice show markedly variable free-running periods. Two wild-type copies of either mCry1 or mCry2 are sufficient to establish a stable free-running clock. A subset of mCry1-/mCry-; mCry2-/mCry2- mice have a diurnal activity preference, suggesting that cryptochromes function in light-dependent behavioral masking. We also analyzed mice lacking both cryptochromes and carrying the homozygous rd retinal degeneration mutation. These mice have markedly depressed behavioral photoresponses in light-dark conditions, despite having an anatomically intact retinohypothalamic tract and normal expression of melanopsin. These results suggest that, similar to insect cryptochromes, mammalian cryptochromes function pleiotropically in both circadian rhythm generation and in photic entrainment and behavioral responses.

Original languageEnglish (US)
Pages (from-to)181-203
Number of pages23
JournalJournal of Neurogenetics
Volume16
Issue number3
DOIs
StatePublished - Jul 2002
Externally publishedYes

Fingerprint

Cryptochromes
Circadian Rhythm
Running
Light
Periodicity
Retinal Degeneration
Circadian Clocks
Drosophila
Insects
Alleles
Mutation

Keywords

  • Circadian rhythms
  • Cryptochrome
  • Entrainment
  • Masking
  • Retinal degeneration
  • Retinohypothalamic tract

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics
  • Neuroscience(all)

Cite this

Van Gelder, R. N., Gibler, T. M., Tu, D., Embry, K., Selby, C. P., Thompson, C. L., & Sancar, A. (2002). Pleiotropic effects of cryptoChromes 1 and 2 on free-running and light-entrained murine circadian rhythms. Journal of Neurogenetics, 16(3), 181-203. https://doi.org/10.1080/01677060215306

Pleiotropic effects of cryptoChromes 1 and 2 on free-running and light-entrained murine circadian rhythms. / Van Gelder, Russell N.; Gibler, Therese M.; Tu, Daniel; Embry, Kris; Selby, Christopher P.; Thompson, Carol L.; Sancar, Aziz.

In: Journal of Neurogenetics, Vol. 16, No. 3, 07.2002, p. 181-203.

Research output: Contribution to journalArticle

Van Gelder, Russell N. ; Gibler, Therese M. ; Tu, Daniel ; Embry, Kris ; Selby, Christopher P. ; Thompson, Carol L. ; Sancar, Aziz. / Pleiotropic effects of cryptoChromes 1 and 2 on free-running and light-entrained murine circadian rhythms. In: Journal of Neurogenetics. 2002 ; Vol. 16, No. 3. pp. 181-203.
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