PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus

Masayuki Ikeda, Takashi Sugiyama, Kaoru Suzuki, Takahiro Moriya, Shigenobu Shibata, Motoya Katsuki, Charles N. Allen, Tohru Yoshioka

Research output: Contribution to journalArticle

13 Scopus citations


Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-β (PLC-β) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-βI and β4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-β4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-βI but not PLC-β4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish (US)
Pages (from-to)907-912
Number of pages6
Issue number5
StatePublished - Apr 7 2000


  • Body temperature
  • Calcium imaging
  • Carbachol
  • Circadian rhythm
  • Immunostaining
  • Locomotor activity
  • Muscarinic acetylcholine receptor
  • Phospholipase C-β
  • Pirenzepine
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Ikeda, M., Sugiyama, T., Suzuki, K., Moriya, T., Shibata, S., Katsuki, M., Allen, C. N., & Yoshioka, T. (2000). PLC β4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. NeuroReport, 11(5), 907-912.